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《復(fù)方褐毛甘西鼠尾對大鼠離體心肌缺血再灌注損傷的保護(hù)作用.doc》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫。
1、復(fù)方褐毛甘西鼠尾對大鼠離體心肌缺血再灌注損傷的保護(hù)作用作者：段為鋼，李惠蘭，張榮平，蘇彥宏，鄭春蘭【關(guān)鍵詞】心肌缺血/再灌注損傷；，，保護(hù)作用；，，褐毛甘西鼠尾摘要：目的與復(fù)方丹參注射液比較，探討及其在抗心肌缺血再灌注損傷方面替代丹參的可行性。方法采用離體Langendorff缺血再灌注模型，低灌15min復(fù)灌30min。實(shí)驗(yàn)后檢測灌流液中MDA含量及LDH,CK，SOD,GSHPx活性，實(shí)驗(yàn)過程全程記錄冠脈流量、心率和心肌收縮力。結(jié)果復(fù)方褐毛甘西鼠尾注射液能顯著降低離體缺血再灌注后MDA,CK,LDH水平，升高SOD和GSHPx,且作用強(qiáng)度相似于復(fù)
2、方丹參；褐毛甘西鼠尾復(fù)方注射液能明顯增加冠脈流量，增強(qiáng)再灌注期間的心肌收縮力；此兩藥均減慢缺血再灌注過程。結(jié)論復(fù)方褐毛甘西鼠尾有較好的抗心肌缺血再灌注損傷作用，褐毛甘西鼠尾可在抗心肌再灌注損傷方面替代丹參。其機(jī)制可能與抗氧自由基損傷有關(guān)。關(guān)鍵詞：心肌缺血/再灌注損傷；保護(hù)作用毛甘西鼠尾TheProtectiveEffectofCompoundedlnjectionMadefromSanqiandHemaoganxishuwEiNativetoYunnanonlsolatedMyocardiallschemia/ReperfusioninRatsAbs
3、tract:Objecticardialprotectionidedinjectionsmadefhemia/reperfusionimodelinvitrowasinttheheartatalowflowtionforl5minatfirsalperfusionfor30mitentsofmalEIcdialdtivitiesoflactatedcreatinekinase(CK)se(SOD),andglutathPx)intheflowofeff1on.ContractileforcdrhythmwererecordeResultsInject
4、ionofreasethecontentsofeactmctiesofSODandheeffectwassimilarnjectionofSPM+PNwaveToinvestigatethenducedbythecompounromSPM+PNduringiscnratsinLangendorffroduced,weperfusedratewithKreb1ssolut,thenfollowednormn.Weexaminedtheconehyde(MDA)andtheacehydrogenase(LDII),,superoxidedismutaio
5、neperoxidase(GSHuentafterreperfusie,perfusionflow,andduringexperiment.SPM+PNwasabletodecMDA,CK,LDH,increasGSH-Pxinvitro,andttothatofSM+PN；Theisabletoenhanceventrce,increasecoronaricalcontractileforyflow,andslackenheartrate.ConclusionThecompoundedinjectionfromSPM+PNpossessescard
6、ialprotectiveeffectagainstischemia/perfusioninjury,whichisnot1esspowerfulthantheinjectionfromSM+PN，andwhichsuggestsSPMcanbealikelyreplacerforSMtoprotectmyocardiumfromischemia/reperfusioninjury.Themechanismsofwhichmaybeassociatedwithdeactivatingoxygenfreeradicles.Keywords：Ischem
7、ia/reperfusioninjury；Protection；S.przewalskiiMaxim.Var.mandarinorumstib.；Panaxnotoginseng(Burk.)中藥丹參Salviami11iorrhizaBunge，SM和三七Panaxnotoginseng(Burk.),PN是中國傳統(tǒng)的活血化癖■中藥，常一起配伍使用用于缺血性心肌病的治療。前期研究表明:褐毛甘西鼠尾注射液在體內(nèi)外均能顯著抑制ADP、血小板活化因子、花生四烯酸誘導(dǎo)的血小板聚集[1];增加離體心臟冠脈流量；對抗心肌缺血引起的紅細(xì)胞壓積升高，加快血流速度，
8、增加毛細(xì)血管開放數(shù)目，改善微循環(huán)、血液流變學(xué)和血流動(dòng)力學(xué)特征[1];減輕心肌缺血時(shí)超微結(jié)構(gòu)的病理變化，減少心
