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1、AbstractWefoundthatEDARCDSconsistedof1389basepairnucleotideswhichencodesa462aminoacidprotein,ithasasignalpeptides,aTumornecrosisfactorreceptorregion,atransmembraneregionanddeathdomain.Thealignmentresultshowsthatthecarpsharesthehighestsimilarity(88.31%)withthezebrafish.However,ithasth
2、erelativelowersimilaritywithXenopuslaevis(64.88%)chicken(63.79%),human(64.36%),mouse(63.50%)duetoevolutionarilydistantrelationships.The5'-UTRsharesthehighvariation,evenwiththemedaka(24.73%)andzebrafish(23.78%).Alsointhe3'-UTRvariessignificantlywithmedaka(35.49%).LetalonewithXenopusla
3、evis(11.73%and9.42%),human(6.50%and8.05%),mouse(10.04%and9.37%).Thein-situhybridizationresultsshowthatthetwogenesuniqueexpressionsignalwasdetectedinskinmatrixwhichthescaleformed,howeverweakornoexpressionsignalwasdetectedinotherpositionsonskin,laterdisappearedinfulldevelopedscalebody,
4、alloftheseresultsshowthatthisgenemayinvolvetheinitiationofthescalesnotthemaintenanceofthescalepatternformation.Keywords:carp,scales,RACE,in-situhybridizationIV目錄目錄摘要.......................................................................................................................
5、...........................IAbstract...........................................................................................................................................III目錄........................................................................................................
6、.........................................V第一章引言.....................................................................................................................................11.1魚類的鱗................................................................................................
7、............................11.1.1鱗的分類.................................................................................................................11.1.2魚鱗的發(fā)育模式......................................................................................................11.2鱗被發(fā)育過程研究進展....
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