資源描述：
《白藜蘆醇苷在大鼠體內(nèi)的藥代動(dòng)力學(xué)研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、第四軍醫(yī)大學(xué)碩士學(xué)位論文to0.25mLplasma.Thesampleswereextractedtwicewith2.0mLacetidin.Theorganicphasewastransferredtoacleantubeandevaporatedtodrynessat50℃underagentlestreamofnitrogenintheventilationcabinet.Theresiduewasdissolvedin50μLofmethanoland10μLwastakenforLC/MS/MSanalysis.(2)0.2mLglucuronidases
2、olution(2000U/mL)wasaddedto0.25mLplasmaandincubatedat37℃for24hinwaterbathshake.Thesamplewastreateddetectedaccordingtostep(1).3.Themodelofintestinaltractperfusioninsituwassetup.Theintestinaparvaof10cminlengthfromthepoint5cmbelowtheduodenumwasperfusedbyintubation,andthentheperfusatesamples
3、werecollectedatdifferentpoints(10,20,30,40,50,60min)viaportalvein.TheconcentrationofresveratrolandpolydatinweredetectedbythemethodofLC/MS/MS.Atthesametime,thesamplewastreatedwithglucuronidasetodetecttheconcentrationofglucuronidatedresveratrolandpolydatin.4.Themodelofliverperfusioninsituw
4、assetup.Thedrugwaspouredintoportalvein,andthentheperfusatesampleswerecollectedatdifferentpoints(10,20,30,40,50,60min)viainferiorvenacave.TheconcentrationofresveratrolandpolydatinweredetectedbythemethodofLC/MS/MS.Atthesametime,thesamplewastreatedwithglucuronidasetodetecttheconcentrationof
5、glucuronidatedresveratrolandpolydatin.RESULTS:1.TaketheratioofpeakareaofpolydatinandresveratroltopeakareaofinternalstandardgenisteinseparatelyforY-axis,andtheconcentrationofpolydatinandresveratrolforX-axistobuilttheworkingcurve.Thetypicalregressionequationtoassaytheconcentrationofthepoly
6、datinintheratplasmawasY=0.0543X-0.032，γ=0.9999，n=6.Thelinearrangewas0.4-200ng/mLandthefixedlowerquantitywas0.4ng/mL.Thetypicalregressionequationtoassay6第四軍醫(yī)大學(xué)碩士學(xué)位論文theconcentrationoftheresveratrolintheratplasmawasY=0.0366X+0.1138，γ=0.9999，n=6.Thelinearrangewas0.4-200ng/mLandthefixedlower
7、quantitywas0.4ng/mL.2.PolydatinwasquicklyabsorbedandbiotransformedinSDrats,andproducedresveratrol,glucuronidatedresveratrolandglucuronidatedpolydatin,etc.AftertheSDratsweregivenatthedoseof50,100and300mg/kgbygavage,theareaundercurve(AUC)ofpolydatinwere53574,85970,228245ng?
