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《三聚氰胺的遺傳毒效應(yīng)及牛奶中三聚氰胺快速檢測(cè)方法研究》由會(huì)員上傳分享，免費(fèi)在線(xiàn)閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、melaminefromdispersiontoaggregationrapidly,alongwiththecolorchangefromredtoblue(orpurple).Aftertheoptimizationofreactionconditions,thewholeanalysisonlyneedsabout20minincludingthesimplepretreatmentwedesigned.Theanalysiscanbeobservedbythenakedeye,andsimpletooper
2、atewithoutsophisticatedinstruments.Intherawmilk,thepresentlimitofdetectionformelamineis1μMbynakedeyesanddownto375nMbytheinstument.Furthermore,weexploretheinteractionprinciplebetweenmelamineandcitrate-stabilizedAuNPsinthiswork;establishthefoundationformodifying
3、theAuNPsandoptimizingthesystemofAuNPs-melamineinthefuture.Inthispaper,wealsoreportarapidandsensitivecolorimetricsensorformelaminedetectioninmilkbytheaptamerbasednanoparticles(AuNPs).AptamercanabsorbonthesurfaceofAuNPsandelectrostaticallyinteractswithPoly(diall
4、yldimethylammoniumchloride)(PDDA),whichprotectsAuNPsfromaggregating.Theintroductionofmelaminewouldformamelamine-aptamercomplexbyhydrogenbonds,thusthefollowingcationicpolymercouldaggregateAuNPsandcausearemarkablechangeincolor.Accordingtothis,melaminecanbedeterm
5、inedbynakedeyesandmeansofabsorbance.Thissensorisselectivefordetectionofmelamineinmilksamplesandhasalimitofdetectionof34nM.Sinceaptamer-modifiedAuNPsismorestabilizedandselectivethanunmodifiedAuNPsinthesensorofmelamine,wehopethatthisdetectionmethod-buildingproce
6、sswillbehelpfulforthedetectionofothersmallmolecules.Keywords：melamine，genetictoxicity，AuNPs，aptamer，detectionVIII萬(wàn)方數(shù)據(jù)目錄摘要...........................................................................................................................................
7、.......VABSTRACT..................................................................................................................................VII圖表清單...........................................................................................................
8、............................XII縮略表..........................................................................................................................................XIV第一章緒論
