資源描述：
《SUMO1、SENP1調(diào)控低氧誘導(dǎo)因子1α在低氧性肺動(dòng)脈高壓發(fā)病中的作用》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、·polymerasechainreaction(RT-PCR)andinsituhybridizationwereusedtodeterminethemRNAexpressionofSUMO-1andSENP-1.ImmunohistochemistryandWesternblotwereadoptedtodeterminetheproteinexpressionofSUMO-1andSENP-1.Co-ImmunoprecipitationwereadoptedtodetermineSUMOylationofHIF-1αbySUMO-1.(2)Thirty-sixpa
2、tientsweredividedintothreegroups:chronicobstructivepulmonarydisease(COPD)withpulmonarypertension(PH)group(PHgroup),COPDwithoutPH(COPDgroup)andcontrolgroup.Theirlungtissueswerecollectedfromsurgicallyresectedspecimens.TheHPSRwasobservedwithmorphmetricanalysistoo.TheexpressionofHIF-1α,VEGF,S
3、UMO-1andSENP-1inpulmonaryarterioleswasexaminedbyinsituhybridizationandimmunohistochemistry.TheSUMOylationofHIF-1αbySUMO-1wasexaminedbyCo-Immunoprecipitation.Results(1)mPAPinhypoxicratsincreasedsignificantlyafter7dofhypoxia,reacheditspeakafter14dofhypoxia,andthenremainedstable.Thehypoxicra
4、tsdevelopedHPSRafter7dofhypoxia,andmoresignificantlyafter14dofhypoxia.RVHIinhypoxicratswasmarkedlyincreasedafter14dofhypoxia.HIF-1αmRNAispositivelystainedincontrolandincreaseddramaticallyafter14dofhypoxia.HIF-1αproteinwaspoorlypositiveincontrol,up-regulatedmarkedlyafter3dand7dofhypoxia,an
5、dthendeclinedslightlyafter14dand21dofhypoxia.VEGFmRNAandproteinwerepoorpositivelystainedincontrol,increasedmarkedlyafter7dofhypoxia,andthenreachedtheirpeakafter14dofexposuretohypoxia.SUMO-1mRNAandproteinexpressioninpulmonaryarteriolewallswasmarkedlyincreasedinpulmonaryarteriolewallsafter3
6、dofexposuretohypoxia,reacheditspeakafter14dofhypoxia,thenweakenedafter21dofhypoxia,butwasstillhigherthanthatinthecontrol.SENP-1mRNAandproteinwerepositivelystainedincontrol.SENP-1mRNAexpressionhadlittlechangesafterexposuretohypoxiacomparedwiththecontrol,however,SENP-1proteinexpressionwasin
7、creasedSlightlyafter3dofhypoxia,anddeclinedgraduallyafter7dofhypoxia.TheresultsofCo-immunoprecipitationshowedthatSUMOylationofHIF-1aalmostdidnothappeninlungtissuesofcontrolrats.Butitwasmarkedlypositiveafter3and7dofhypoxiainlungtissuesofrats,andreacheditspeakafter14d
