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1、蕎麥花葉總黃酮對(duì)壓力負(fù)荷大鼠腦神經(jīng)遞質(zhì)的影響儲(chǔ)金秀韓淑英朱麗莎白靜楊瑞嫻（華北煤炭醫(yī)學(xué)院藥理學(xué)教研室唐山063000）【摘要】目的探討蕎麥花葉總黃酮（TFBFL）對(duì)壓力超負(fù)荷大鼠腦內(nèi)神經(jīng)遞質(zhì)5-羥色胺（5-HT）、去甲腎上腺素（NA）及乙酰膽堿（Ach）的影響和意義。方法藥物干預(yù)組分別予TFBFL高劑量（0.4g·kg-1·ｄ-1）、低劑量（0.2g·kg-1·d-1）、卡托普利（60mg·kg-1·d-1）灌胃（ig），模型對(duì)照組和假手術(shù)組給予等量的生理鹽水，每日1次(o.d.)，連續(xù)給藥1周后
2、用銀夾造成腹主動(dòng)脈狹窄從而導(dǎo)致大鼠心臟超負(fù)荷，于手術(shù)后1小時(shí)、3小時(shí)、12小時(shí)立即取腦組織，用分光光度法測(cè)定腦組織內(nèi)神經(jīng)遞質(zhì)NA、5-HT和Ach的含量。結(jié)果手術(shù)后1小時(shí)、3小時(shí)、12小時(shí)模型對(duì)照組5-HT及NA的含量相對(duì)于正常對(duì)照組均有較顯著的升高（P<0.05，P<0.01），升高的程度隨手術(shù)時(shí)間的延長而增加。TFBFL高低劑量均能降低5-HT和NA的含量，以高劑量作用更強(qiáng)。各組Ach含量改變不顯著。結(jié)論TFBFL對(duì)壓力超負(fù)荷大鼠腦內(nèi)Ach的影響不明顯，但能顯著降低5-HT和NA的含量，這可能
3、是TFBFL抑制心肌肥厚的機(jī)制之一?！娟P(guān)鍵詞】蕎麥；總黃酮；5-羥色胺；去甲腎上腺素；乙酰膽堿；心肌肥厚EffectsofTotalFlavonesofBuckwheatFlowersandLeavesontheNeurotransmittersinBrainsofPressureOverloadRatsChuJinxiu，HanShuying,ZhuLisha,BaiJing,YangRuixian（DepartmentofPharmacology,NorthChinaCoalMedicalCo
4、llege,Hebei,China063000）【Abstract】ObjectiveToexploretheinfluenceandsignificanceoftotalflavonesofbuckwheatflowersandleaves(TFBFL)ontheneurotransmittersi.e.5-hydroxytryptamine(5-HT),noradrenaline(NA),acetylcholine(Ach)inbrainsofpressureoverloadrats.Meth
5、odsRatsweregivenTFBFL(0.4g·kg-1·ｄ-1、0.2g·kg-1·d-1)andcaptopril（60mg·kg-1·d-1）byintragastricadministration（ig）indruginterferencegroupsoncedaily(o.d.)for1week,meanwhile,normalsalineofthesamevolumewasadministratedtotheratsinmodelcontrolgroupandsham-opera
6、tedgroup.Then,cardiacpressureoverloadmodelwassetbycoarctatingtheabdominalaortawithasilverclip.Thebrainswerecollectedat1h,3hand12hafteroperationandhomogenated,andthecontentsof5-HT,NAandAchweredetectedwiththespectrophotometricmethod.ResultsComparedwithn
7、ormalcontrolgroup,thecontentof5-HTandNAinmodelcontrolgroupat1、3、12hoursafteroperationincreasedremarkably（P<0.05，P<0.01）andshowedtimedependence.TFBFLatthetwodosagescouldbothlowerthecontentof5-HTandNAandshoweddosagedependence.However,thecontentofAchhadn
8、osignificantchangeinbothmodelanddrugtreatmentgroups.ConclusionTFBFLhadlittleeffectonAchinbrainsofpressureoverloadrats,buttheycouldremarkablydecreasethecontentof5-HTandNA,whichmightbeoneofthemechanismsTFBFLinhibitedmyocardialhypertrophy.【Keywor
