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1、第三軍醫(yī)大學(xué)碩士學(xué)位論文ThevaluestudyofPD-L1expressionandgenepolymorphismofthediagnosisandprognosisingastriccancer.AbstractBackgroundandobjectiveGastriccancer(GC)isoneofthemostcommonfatalcancers,Theincidenceforgastriccarcinomahashadarecentincrease.Effectivemethodsforearlydiagnosis,m
2、onitoringmetastasis,andprognosisarecurrentlyunavailableforgastriccancer.Immunotherapyhasshownbrightpromiseinthetreatmentofgastriccancer.Unfortunately,itwasreportedthatgastriccancercellScanescapeimmunesurveillancebyavarietyofmechanismswhichpreventtheimmunesystemfromproduc
3、ingeffectiveantitumorimmuneresponses.SomestudieshavereportedthatPD-L1increasesapoptosisofantigen—specifichumanT-cellclonesandinhibitstheactivationofCD4andCD8T-cellsinvitro.MoreoverPD—L1highexpressioninthemultipletumor,includinggastriccancer.SeveralstudieshaveshownthatPD·
4、-1orPD··L1antibodiescouldrelievetheinhibitoryeffectsofPD-L1oncytotoxicT-cellsandhenceacceleratetheremovaloftumorcellsbycytotoxicT-cellsandinterruptingthePD1/PD·L1signalpathwayisapromisingtherapeuticstrategyforcancer.Apurinic/apyrimidinicendonuclease1(APE1)playsanimportan
5、troleinmultiplebiologicalprocesses。ItcanrepairDNAdamageandsuppresstumorigenesisthroughmaintaininggenomestability.APE1,asaredox—activeprotein,alsoactivatesmanytranscriptionfactors.APE1overexpressionenhancedthetumorigenesisofgastriccancer.SNPisoneoftheimportantfactorsforca
6、ncersusceptibility.SeveralpolymorphismsinexonsofPD.Llhavebeendescribedandrelatedwithautoimmunediseases.However,theSNPofPD—L1forgastriccanceralsorarelyreported.Inpartone,wedetectedtheassociationbetweentheexpressionofPD—L1andAPElwiththegastriccancerpatientswith107cases.And
7、thenThePCR—CTPPwasconductedtoanalyzetheassociationsofgastriccancersusceptibilitywithPD—L1genesinglenucleotide2萬方數(shù)據(jù)第三軍醫(yī)大學(xué)碩士學(xué)位論文polymorphism.ToprovidetheoreticalsupportforthePD—L1asatargetinimmunetherapeuticofgastriccancer,MethodsThisprogramconsistedoftwoparts.Inthepartone
8、,theexpressionofPD—L1andAPElin107gastriccancertissueswereevaluatedbyIHC.Observetheexpressionofthesetwog