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1、ClassifiedIndex:Code:10224Confidential(yes/no):noNO.:120410276DissertationfortheMasterDegreeGeneticAnalysisandGeneMappingofYe//owLeafCurlVirusResistantGene咖.5‘T0matoin加一5’.01matoCandidate:YangHuanhuanSupervisor:ProfessorLiJingfuDegreeCategory:MasterofAgricultureCollege:CollegeofHorticult
2、ureFirstleveldiscipline:HorticultureSecondleveldiscipline:OlericultureHarbinChinaJune2015萬方數(shù)據(jù)目錄㈣㈣㈣㈣刪㈣刪0Y2771679目錄摘要I英文摘要IIIl引言一11.1研究的目的和意義.11.2國內(nèi)外研究進展.11.2.1TYLCV的研究進展.11.2.2TYLcv及其傳播一21.2.3番茄黃化曲葉病毒(TYLCV)接種及抗性鑒定21.2.4番茄黃化曲葉病主要防治方法.31.2.5抗番茄黃化曲葉病育種一41.2.6分子標記技術51.2.7抗病品種的選育81.3研究內(nèi)容、技術路線
3、.92材料與方法112.1試驗材料l12.1.1番茄材料l12.1.2試驗試劑.112.1.3主要儀器與設備.112.2.1試驗設計.112.2.2番茄黃化曲葉病毒病抗性鑒定122.2.3DNA提取及抗感池構(gòu)建132.2.4ty一5基因SSR連鎖群的構(gòu)建及染色體初定位.142.2.5數(shù)據(jù)統(tǒng)計及連鎖距離的測定152.2.6番茄黃化曲葉病毒種質(zhì)資源的抗病性鑒定153結(jié)果與分析173.1遺傳規(guī)律分析173.1.1番茄黃化曲葉病抗性遺傳分析173.1.2各世代發(fā)病的株問分布173.2基因組DNA的提取183.3SSR分子標記結(jié)果】93-3.1SSR引物的篩選一193.3.2抗、
4、感池的構(gòu)建.193-3.3F2代單株的SSR標記203.4連鎖關系作圖20萬方數(shù)據(jù)東北農(nóng)業(yè)大學農(nóng)學碩士學位論文3.4.1SSR標記的遺傳連鎖分析.203.4.2連鎖關系作圖.203.5番茄黃化曲葉病毒病抗病性鑒定..224討論..244.1抗病性鑒定244.2抗病基因的分子標記244.3番茄抗TvLcv種質(zhì)資源的篩選和品種的選育.255結(jié)論26致謝27參考文獻28附錄36攻讀碩士學位期間發(fā)表的學術論文萬方數(shù)據(jù)CONTENrSCoNTENTSAbstractinChineseIAbstractinEnglish?!ぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁぁ?/p>
5、···································-··················-···············‘IlI1Introduction······························-·····-····-···-·······-··-···············-···········-····-···-··························。?!!??!?1.1PurposeandSignificanceofThisResearch··············-‘‘‘11.2Summa
6、ryofDocument’·······-·‘-‘·‘-···········-·······-··‘‘··‘···‘·-‘-··‘·-·····_-···-······-········--······-·······-···--·‘_····‘11.2.1TheResearchofTomatoYellowLeafCurlViruls‘11.2.2IntroductionofTomatoYellowLeafCill"lVirus一21.2.3InoculatingandDentificationofTomatoYellowLeafCurlVirus?!ぁぁぁ····
7、·······t··············-········‘21.2.4PreventionMeasureofTomatoYellowLeafCLIrlVirus···-··。31.2.5ScreeningofResistanceSourcesandInheritanceofResistance··41.2.6MolecularMarkerandResistantbleBreeding-”51.2.7ResistantbleBreeding‘··_······-···············-···············-·····