酵母菌株zz-46利用木質(zhì)纖維素水解液產(chǎn)油脂的研究

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1、酵母菌株ZZ-46利用木質(zhì)纖維素水解液產(chǎn)油脂的研究李斌劉旭敏肖澤濤韓堯王冬梅郭書賢*（河南省工業(yè)微生物資源與發(fā)酵技術(shù)重點(diǎn)實(shí)驗(yàn)室；南陽理工學(xué)院生物與化學(xué)工程學(xué)院，南陽473004)摘要利用小麥秸稈和甘蔗渣制備木質(zhì)纖維素水解液，確立了可行的脫毒方法，研究酵母菌株TrichosporondermatisZZ-46在脫毒水解液與未脫毒水解液中的發(fā)酵產(chǎn)油能力，并采用搖瓶發(fā)酵培養(yǎng)，對(duì)菌株發(fā)酵過程、產(chǎn)油能力、發(fā)酵動(dòng)力學(xué)以及油脂脂肪酸組成進(jìn)行了探索。結(jié)果表明：采用氫氧化鈣與活性炭混合脫毒法最佳，此時(shí)糠醛毒性物質(zhì)含

2、量最低，僅為0.0322?μg/mL，ZZ-46利用該脫毒水解液發(fā)酵產(chǎn)油較高，120?h時(shí)為ZZ-46最佳培養(yǎng)時(shí)間，生物量、油脂產(chǎn)量、油脂含量分別為18.502?g/L、6.793?g/L、36.71%。發(fā)酵動(dòng)力學(xué)分析表明：ZZ-46的底物消耗與菌體生長(zhǎng)同步，屬于與菌體生長(zhǎng)相關(guān)型，而油脂積累則屬于與菌體生長(zhǎng)部分相關(guān)型。不飽和脂肪酸含量較高是該油的特點(diǎn)。這對(duì)發(fā)酵木質(zhì)纖維素水解液產(chǎn)油脂的研究具有重要意義。關(guān)鍵詞木質(zhì)纖維素TrichosporondermatisZZ-46產(chǎn)油脂能力動(dòng)力學(xué)參數(shù)分析中圖分類

3、號(hào)：TS222文獻(xiàn)標(biāo)志碼：A文章編號(hào)：20180121StudyontheUseofLignocelluloseHydrolysateFermentationforOilbyYeastZZ-46LIBinLIUXuminXIAOZetaoHANYaoWANGDongmeiGUOShuxian*(HenanKeyLaboratoryofIndustrialMicrobialResourcesandFermentationTechnology;SchoolofBiologicalandChemical

4、Engineering,NanyangInstituteofTechnology,Nanyang473004)AbstractThistopicusingwheatstrawandbagassehydrolyzedwithrawmaterialpreparation,onthebasisoftheestablishedthepossiblemethodofdetoxification,studiedTrichosporondermatisZZ-46inhydrolysatehydrolyzedan

5、ddetoxificationoffermentationcapacity.Usingtheshakingflaskfermentationcultivation,thefermentationprocess,capacityandthefermentationkineticparameterswereanalysied.Theresultsshowedthatthecalciumhydroxidemixedwithacticarbonwasthebestdetoxificationmethod.

6、Thecontentoffurfuralwasthelowest,only0.0322?μg/mL.TrichosporondermatisZZ-46useddetoxifiedlignocellulosehydrolysatetoproducehighoilproduction.Theresultsshowedthatthe120hasthebesttrainingtime,andthecoefficientofbiomass,oilproduction,oilcontentwere18.502

7、?g/L,6.793?g/Land36.71%.FermentationkineticsanalysisshowedthatTrichosporondermatisZZ-46ofthesubstrateconsumptiongrowthsynchronizationwithbacteria,belongtothetypewasrelatedtothegrowthofthebacteria,andtheoilaccumulationtypebelongedtothepartrelatedtotheg

8、rowthofthebacteria.Thecharacterofoilisthehighcontentofunsaturatedfattyacid.Thestudyoflignocellulosehydrolysisfermentationtoproduceoilisofgreatsignificance.KeywordsLignocellulosic,TrichosporondermatisZZ-46,Oilproductioncapacity,Fermentationkine