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《3-大豆苷元磺酸鈉對氧化低密度脂蛋白致內(nèi)皮細(xì)胞損傷的保護(hù)作用研究》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫。
1、3-大豆昔元磺酸鈉對氧化低密度脂蛋白致內(nèi)皮細(xì)胞損傷的保護(hù)作用研究Sodium3-soyasodiumsulfonateistheinnerskinoftheLDLProtectiveeffectofcellinjuryTheauthor:jingjing,huangyuping,huangyushan,wangshu-people【abstract】objectivetostudy3J-soybeansulfonicacidsodium(DSS)toinduceumbilicalveininoxidizedlow-densitylipoproteinTheprotectivee
2、ffectofendothelialcel1injuryanditsmochanism?MethodstheendothelialcellsofthenavelveinwereculturedinvitroInthecontrolgroup,thecontrolgroup(ox-ldl)andoxidativedamagewereaddedtothevitaminEcontrolgroup(VE+ox-ldl),oxidativedamageisaddedtoDSSlow,middle,highconccntrationgroup(dss-1,dss-manddss-h)Gro
3、up).Theeffectoftheoxy-ldleffectontheendothelialcellsthatpreloadedtheVEandthedifferentconcentrationsofDSSto24h,Continuctotrain24h,MTTmethoddetectscellvitality,colorimetrydetectspropylenedialdehyde(MDA)andnitricoxide(NO)Thecontentoftheenzymelactatedehydrogenase(LDH),superoxidedismutase(SOD),ni
4、tricoxidesynthase(NOS)andGlutathioneperoxidase(gsh-px)activity?Theresultsarepre-addedtotheDSS,whichhasahighconcentrationThevitalityofthedamagedendothelialcellscansignificantlyreducethereleaseofMDAandNO,whichdamageendothelialcellsTheactivityofLDH,NOS,SOD,andgsh-pxofdamagedcellswasenhaneed,but
5、lowconcentrationsofDSSwereusedThestandardhaslittleeffect?ConclusionDSScanreducetheoxidativedamageofoxandLDLtovascularskincellsOxidativeprotection.[keywords]3'-soysulfonate;LDL?Theendothelialcells;ProtectiveeffectAbstract:ObjectiveToinvestigatetheprotectiveeffectandMechanismsof3'-daidzeinsulf
6、onatesodiumonvascularendothelialCellinjuryinducedbyoxidizedlowdensitylipoprotein,MethodsHumanUmbilicalveinendothelialcells(HUVECs)wereculturedinvitroandDividedintofivegroups:control,ox,LDL,dss-1.dss-mandDSS-Hgroups.Endothelialcellswereincubatedwithox-LDLfor24HoursaftertheywereincubatedwithVi
7、taminE(VE)anddifferentTheproliferationofHUVECswasassayedbyThe3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny1tetrazoliurabromideMethod,andthelevelsofNOandMDA,theactivitiesofLDH,SOD,NOSTheyweremeasuredbythelevelofMDAandtheactivityoftheactivityLDHinoxy-ldld
