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1、雙氫青蒿素對前列腺癌裸鼠種植瘤生長及VEGF表達的影響李慶春高小玲石之虎鄒聰羅子國（重慶醫(yī)科大學電鏡室，重慶400016）摘要目的研究雙氫青蒿素（DHA）對前列腺癌PC-3細胞裸鼠種植瘤的抑制作用及對VEGF表達的影響，并探討其作用機制。方法采用人前列腺癌PC-3細胞建立裸鼠種植瘤模型，20只模型鼠隨機分為對照組、溶劑組、DHA大劑量組及DHA小劑量組，經(jīng)13d干預后，計算抑瘤率；光鏡及電鏡觀察腫瘤組織形態(tài)學表現(xiàn)，免疫組織化學法檢測VEGF的表達水平，體視學分析腫瘤微血管密度（MVD）。結果DHA大小劑量組抑瘤率分別為69.221%和63.186%，腫瘤組織內(nèi)凋亡細
2、胞明顯增多，可見典型的凋亡小體；免疫組化結果顯示，DHA大小劑量組VEGF表達及MVD均低于對照組（P＜0.05）。結論DHA具有較強的抗腫瘤作用，其機制與下調凋亡相關基因VEGF有關。關鍵詞:雙氫青蒿素；前列腺癌；凋亡；VEGF；微血管密度中圖法分類號：R284.1;R979.1文獻標識碼：ATheeffectsofdihydroartemisininontransplantedprostatecancerinnudemiceandexpressionofVEGFLIQing-chun,GAOXiao-lin,SHIZhi-hu，ZOUCong,LUOZi-guo
3、*(LaboratoryofElectronMicroscope,ChongqingMedicalUniversity,Chongqing400016,China)[Abstract]Objective:Tostudytheinhibitoryeffectsofdihydroartemisinin(DHA)onthegrowthoftransplantationhumanprostatetumorPC-3cellsinnudemiceandtheexpressionlevelofVEGFandexploretheunderlyingactionmechanismof
4、DHA.Methods:ProstatecancerPC-3cellsweretransplantedinto20nudemicetoestablishthesolidtumormode.Thesenudemicewererandomlydividedinto4groupswith5miceineachgroup:controlgroup,solventgroup,largedoseDHAgroupandlowdoseDHAgroup.Thetumorgrowthinhibitionratewascalculatedonday13afterdrugadministr
5、ation;PathomorphismchangesofPC-3cellswereobservedbylightmicroscope(LM)andtransmissionelectronmicroscope(TEM)afteradministrationofDHA.TheproteinexpressionofVEGFwastestedbyimmunohistochemicalmethod;作者簡介：李慶春，河北省張家口市人，男，醫(yī)師，主要從事前列腺癌方面的研究。電話:13658380331，E-mail:695786349@qq.com通信作者：羅子國，電話：（02
6、3）68485838，E-mail:luoziguo@cqmu.edu.cn收稿日期：08-07-11修回日期：08-08-31Micro-vasvulordensity(MVD)wasanalysedbystereologicalmethod.Results:ThetumorgrowthinhibitionratesoflargedoseDHAgroupandlowdoseDHAgroupwere69.221%and63.186%;ApoptoticcellsweresignificantlyincreasedandTEMexaminationrevealedth
7、atthescatteredapoptoticbodieswereobservedintumortissuesofDHAgroups;ImmunohistochemicalexaminationrevealedthattheproteinexpressionofVEGFwasdecreasedinDHAgroups（p＜0.05）;MVDwassignificantlydecreasedinDHAgroups（p＜0.05）.Conclusion:TheseresultsdemonstratedthatDHAhasstrongerinhibitoryeffect
