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1、溫州醫(yī)學(xué)院碩士學(xué)位論文AzithromycininducedapoptosisoftheasthmaticratsthroughthemitochondriapathwayPostgraduate：YinJuanDirector：Prof．DaiYuan—tongDir．WuCheng-yun，Dir．XiaXiao-dongAbstractOBJECTIVE：1．ToobservechangesofthemorphologyandchromatinDNAofairwaysmoothmusclecellsinvitr02．Toobservechangesofmi
2、tochondrialmembranepotential，andtheexpressionofcytochromeCandcleavedcaspase-3inairwaysmoothmusclecells．3．ToinvestigatetheinfluenceofazithromycinontheopoptosisofASMCsandthemitochondriapathway，andtoexploreitspossiblemechanisms．METHODS：20maleSprague-Dawleyratswererandomlydividedintofour
3、groupsonaverage，includingcontrolgroup(groupC)andasthmagroup(groupA0)．Inthisexperimenttheratmodelofchronicasthmawasexploited，inwhichratsweresensitizedwithovalbuminandAI(OH)3，andrepeatedlyexposedtoaerosolizedovalbumin．24hoursafterthelastchallenge，theseratswereaneshetizdwithanintraperit
4、ionealinjectionof10％chloralhydrateandweresacrificed．AirwayremodelingwasobtainedbymeasurementandcalculationofWat(totalbronchialwallarea)／Pbm(basementmembraneperimeter)andWam(smoothmusclearea)／Pbmundermicroscope．Thesmoothmusclecellswereisolatedbyprimary—explantmethodfromthebronchialtre
5、einvitro，andbetweenpassages3and8wereusedforexperiments．BronchialsmoothmusclestripswereculturedinvitroinducedbyAZMat0．1or100pg／m1．Growthactivityassaywasdetectedbycck8，thedegradationofASMCschromatinDNAwereanalyzedbyagarosegelelectrophoresis，andthemorphologicalchangeswasobservedunderIas
6、erconfocalmicroscopebystainingwithAnnexinV／PI．JC一1dyewasusedtomeasurechangesinmitochondrialmembranepotential．TheexpressionofcytochromeCandcleavedcaspase一3wasexaminedbywesternblot．RESULTS：1．Theobservationresultsoflightmicroscope：Underlightmicroscope，HE‘stainedlungtissueingroupA0showed
7、inflammatorycellsinfiltratingaroundthebronchialwallandthevascularwall．Therewerealsoevidenceofairwayepitheliaedamage,smoothmusclethickening．Thepathologicalalterationshadnotbeenseenin6溫州醫(yī)學(xué)院碩士學(xué)位論文groupC．2．Imageanalysisforbronchialwallthicknessandthesmoothmusclethickness：WatandWamingroup
8、Cwere31．94士5
