資源描述：
《幾種蟲草無性型真菌菌絲中天然色素的的研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學術(shù)論文-天天文庫。
1、關(guān)鍵詞：粉被瑪利亞霉，雙梭孢隔梭孢，珊瑚蟲草，天然色素，HPLC-MS，HSCCC，穩(wěn)定性IIAbstractAseriesstudieswerecarriedoutonnaturalpigmentsfrommyceliaofthreefungiRCEF4585、RCEF4337、RCEF4022isolatedfromCordycepspruinosaPetch、Septafusidiumbifusisporum、CordycepsmartialisSpeg.,respectively.ThestudiesincludedHPLC-MSanalysisofthecrudeextracti
2、on;isolationofthetargetpigment;identificationofthepigment;stabilitytestsofthepigment.HPLC-MSanalysisshowedthatthenaturalpigmentinmyceliaofRCEF4585wasamixture.WiththehelpofhighresolutionMS,themolecularformulaofthemainpigmentwasidentifiedasC26H32O9.UVanalysisresultsrevealedthatthiscompoundhasultra-v
3、ioletabsorbanceat228nm、260nm、413nm、434nm.ThemainpigmentwaspossiblyanewcompoundaccordingthesearchingresultsofChapman&Hallnaturalproductsdatabase.TherewasonlyonepigmentfoundfromthemethanolextractsofthemyceliaofRCEF3669.AnalysisresultsofHPLC-DAD-MSrevealedthatthemolecularformulaofthepigmentwasC30H18O
4、12,andthattheultra-violetabsorbanceofthepigmentwas225nm、266nm、297nm、467nm.Thepigmentcompoundwasidentifiedasdimericanthraquinone,accordingtotheMsandUVdata.Thispigmentwasthefirsttimeisolatedfromentomogenousfungi.HPLC-MSanalysisshowedthatthenaturalpigmentsinthemethanolextractsfrommyceliaofRCEF4337wer
5、esimilartothosepigmentsfromRCEF4584.However,onedifferentpigmentwithmolecularformulaC30H18O13wasfoundinethylacetateextractsfromRCEF4337.Thepigmenthasultra-violetabsorbanceat225nm、266nm、297nm、503nm.Accordingtomolecularformulaandultra-violetabsorbance,thesearchingresultsofChapman&Hallnaturalproductsd
6、atabaserevealedthatthispigmentwaspossiblyanewcompound.High-speedcounter-currentchromatographywasusedforseparationandpurificationofnaturalpigmentP-1fromthemyceliaofRCEF4585.Thetow-phasesolventsystemwasethylacetate-n-butanol-methanol-water(10:3:3:10).Theupperphasewasstationaryphaseandthelowerphasewa
7、smobilephase.Theflow-rateofthemobilephasewas2.0ml/minandtherotationspeedwas850r/min.TheeffluentofthecolumnwasmonitoredwithaUVdetectorat340nm.AndSemi-PreparationHPLCwasemployedtoseparateandpurifynaturalpigment,tow
