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1、●■上海海洋大學(xué)碩士學(xué)位論文StudyonEffectsofpHstressonPhysiologicalandBiochemicalindexesinthreespeciesofshrimpsABSTRACTWaterisshrimplivingenvironment,SOwaterqualityhaveadirectimpactonthegrowthandeconomicbenefitsofshrimp.InammoniaN,pH,dissolvedoxygen,temperatureandsalinit
2、yphysiochemicalindexs,pHisoneofimportantphyrsiochemicalindexandoneofsynthesisreactionindexofwaterchemical’traits,canaffectwaterbiologicalproductivity.Atpresent,thestudyaboutpHstressonshrimpgrowth,iontransportenzymeandimmunerelatedindexshavereportedinthedome
3、sticandforeign.However,thecomprasionstudyofpHstressinFenneropenaeuschinensis,LitopenaeUSvannamei,Marsupenaeusjaponicushavn’tseenuntilnow.thisthesisselectedpHastheexperimentalchangefactorandstudiedcomprasionofpHstressongrowth,survivalrateandactivityofion—tra
4、nsportenzyme,immunerelatedfactorsinFchinensis.L.vannamei,M.japonicas.Thisstudyincludethreeparts。Thefirstpart:Thesurvivalrate,舀llNr。K,.ATPaseactivities,serumiNOS.PO。SOD,GSH-PX,LSZactivitiesandmuscleRNA/DNAwerecomparedinFchine.nsis.,LvannameiandMjaponicuswhic
5、hwererearedundercontrolgroup(pn8.2),low(pn7.2),andhi曲(pH9.2)pHstressconditionsinthisstudy.Resultsshowedthattherewerenonegativeeffectamongthreespeciesofshrimpswhenrearedinthecontrolgroupfor96h.UnderloworhighpHstressconditions,thesurvivalratesofMjaponicus,L.v
6、annameiandEchinensisfor96hwere96.7%and97.O%。60.1%and54.6%,58.6%and13.3%,respectively.、研threspecttogillNa+-K+-ATPaseactivity,三.vannameishowedhighestlevelandMjaponicuswaslowestundercontrolgroup.Thechangeon百llNd-K十一ATPaseactivityofEchinensisWasthebiggest.w11il
7、ethatofM.japonicaswassmallestunderbothlowandhighpHstressconditions.Also,allthreespeciesofshrimpstendtochangemuchmoreingillNa+.K+一ATPaseactivityunderpH9.2.Inthecontrol,theactivitiesofnonspecificimmunityrelatedenzymesofbothL.vannameiandM.japonicusweresignific
8、antlyhigherthanthatofF-chinensisandPO.Ⅳ●上海海洋人學(xué)碩士學(xué)位論文andSODactivitiesofM.japonicusweremarkedlyhigherthanthatofL.Pannamei.whiletheLSZactivityofL.vannameiwashigherthanthatofMjaponicus.Thechangesinnonspeci