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1、Source:http://www.science-truth.com植物學(xué)通報2005,22(6):703~707ChineseBulletinofBotany實驗簡報沙棘果皮多糖清除氧自由基的活性研究①②張海容(山西省忻州師范學(xué)院生化分析技術(shù)研究所忻州034000)摘要沙棘(Hippophaerhamnosides)果皮經(jīng)80℃恒溫水浴提取,乙醇沉淀得粗多糖。Sevag法去蛋白,2+經(jīng)50%和70%乙醇分級,得3種級分沙棘多糖H1、H2和H3;以Fenton反應(yīng),即H2O2/Fe/水楊酸為.OH產(chǎn)-.生和檢測體系;以鄰苯
2、三酚/EDTA/Tris-HCl為O2產(chǎn)生體系,對沙棘多糖H1、H2和H3進(jìn)行抗氧自由基活-.性研究。結(jié)果表明,沙棘多糖對.OH和O2有較顯著的清除能力。不同級分多糖H1、H2和H3濃度達(dá)200mg.mL-1時,對.OH的清除率分別為44.9%、49.0%和26.4%,抗O-.活性分別為36.9%,15.4%和23.1%。多2糖質(zhì)量濃度增大時,兩種自由基清除率增加,且呈量效關(guān)系。關(guān)鍵詞沙棘,多糖,超氧陰離子,羥自由基,自由基清除率StudyoftheScavengingRadicalActivitiesofPolysacch
3、arideofHippophaerhamnosidesFruitPeel②ZHANGHai-Rong(InstituteofBiochemicalAnalysis,XinzhouTeachersUniversity,Xinzhou034000)AbstractTostudythescaventingradicalactivitiesofpolysaccharideofHippophaerhamnodidesfruitpeel,crudepolysaccharidewasisolatedfromthefruitpeelofH.
4、rhamnosideswithhotwaterat80℃,thenprecipitatedbyethanol,andtheproteinintheextractswasremovedbytheSevagmethod.ThefractionsofH1、H2andH3ofH.rhamnosidespolysaccharidewereobtainedbyadding50%and70%ethanol,respectively.TheFentonreactionwasusedtoproduceanddetecthydroxylradi
5、cals(.OH),andpyrogallol/EDTA/Tris-HClwasusedtoproduceanddetectsuper--.oxideanion(O2).H.rhamnosidespolysaccharideshadastrongscavengingcapacityforboth.OHandO-.oxygenradicals.WhenthefractionalconcentrationsofH、HandHwereat2002123-1mg.mL,theirscavengingratestohydroxylra
6、dicalswere44.9%,49.0%,and26.4%,respectively,andtosuperoxideanion36.9%,15.4%,and23.1%,respectively.Thescavengingratestooxygenradicalsincreasedwithincreasingthepolysaccharideconcentration;thereappearedapropor-tionalrelationbetweeneffectandquantity.KeywordsHippophaerh
7、amnosides,Polysaccharide,Superoxideanionfreeradical,Hydroxylradicals,Freeradicalscavengingrate①山西省歸國留學(xué)基金(2004-67)資助。②通訊作者。Authorforcorrespondence.E-mail:biochem@xztc.edu.cn收稿日期:2005-01-18接受日期:2005-04-30責(zé)任編輯:于昕www.science-truth.comSource:http://www.science-truth.com
8、70422(6)-.氧自由基主要指超氧陰離子(O2)、羥自1.1材料與儀器1由基(.OH)、單線態(tài)氧(O2)和過氧化氫(H2O2)沙棘果皮由山西岢嵐縣酸溜溜廠提供(10一類化學(xué)性質(zhì)活潑、半衰期極短、能奪取其月下旬)。他敏感分子電子的氧化劑。氧自由基過多的DKZ電熱恒溫振蕩水槽,上海一恒實驗
