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1、AbstractHightemperaturereducestheproliferationandoccludinexpressioninratSertoilcellsinvitroObjectiveThepurposeistofurtherexploretheactionmechanismofvarIoustemperaturesonmaleinfertilityorsubfertilityviaa11observationontheeffectofdifferenttemperaturesontheproliferationandocclu
2、ding(OCLN)expressionofinvitroratsertolicells(SOs)．MethodsFirstlv，isolateSCsfromthetesticleofmaleWistarratandtestitbyoilredOsmimngandimmunohistochemistryFasL．Secondly，setupSCsintoonecontrolgroup(34℃)andfiveexperimentalgroups(35℃、36℃、37℃、38℃、39℃)．CultivateSCsundereachtemperatu
3、reforfourdays．Thirdly，tomakeacomparison，applyCCK-8assaytomeasurecellproliferation；observecellmorphologyandstIucnlreafterHematoxylin．Eosinstaimng，underinvertedmicroscopeandlightH1icroscope：thendetermineOCLNexpressionlevelthroughwesternblottingandcellimmunofluorescence．Results
4、AsaresultofoilredOandFasLtest，thepurityofisolatedSCswas96．20士1．95％inthisexperiment．CCK．8assayindicatedthatproliferationofSCsincreaSedasthetemperaturewasrisingbetweentherangeof34℃and36℃，anddecreaSedasthetemperatureWasrisingintherangeof36～39℃．Meanwhile，pyknoficnucleiandfragmen
5、tationofSCsweremoreobviousunderatemperatureabove36℃．Otherwise，westernblottingandimmunofluorescenceshowedapeakofOCLNexpressionlevelunder36℃．Asacontrast，OCLNexpressionleveldecreasedwhilethetemperaturewasgoingupover36℃fP<0．01)．ConelusionsTheresultofthisstudyindicatedthatahigher
6、temperature(37℃，38℃or39℃)inhibitedtheproliferationofratSCsinvitro，anddecreasedtheexpressionofOCLN，fromwhich，wecarltentativelyspeculatethatallighIxtemperatureabove36。CmayreducemalefertilitybyaffectingtheproliferationofSCsandintegrityoftightjunctionbetweenSCsorSCsandothercells
7、．Postgraduatestudent：Hujianting(Urology)Directedby：Prof．Xin—shengWangKeyWords：temperature；Sertoliceil；occludin；blood-testisbarrie：r；spermatogenesis目錄引言?????????????????????????????．．1第1章材料和試劑???????????????????????21．1試驗動物??????????????????????????21．2主要實驗試劑?????????????????
8、???????21．3主要儀器設(shè)備與工具?????????????????????3第2章實驗方法????????????????????????52
