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1、異甘草酸鎂對大鼠肝星狀細(xì)胞增殖及活力影響【摘要】目的觀察異甘草酸鎂對大鼠肝星狀細(xì)胞(HSC)增殖和活力的影響。方法用含不同濃度異甘草酸鎂的培養(yǎng)基(0、0.01、0.1、1.0、10.0mg/ml)培養(yǎng)HSC,通過MTT法和LDH法檢測異甘草酸鎂對HSC增殖和活力的影響,并同時觀察其形態(tài)變化。結(jié)果MTT法測得與空白對照組(0mg/ml)比較,培養(yǎng)基所含的異甘草酸鎂濃度為1.0mg/ml時,對HSC具有明顯的抑制增殖作用;10.0mg/ml時,其抑制增殖作用達(dá)到最高峰。LDH法測得各濃度組的異甘草酸鎂給藥組細(xì)胞上清液中LDH活力無明顯差異。結(jié)論異甘草酸鎂在
2、一定劑量范圍內(nèi)對HSC具有明顯的抑制增殖作用,但對細(xì)胞上清液LDH活力無明顯的影響?！娟P(guān)鍵詞】異甘草酸鎂;HSC-T6細(xì)胞株;增殖;活力EffectsofmagnesiumisoglycyrrhizinateontheproliferationandactivityofrathepaticstellatecellLONGSu-jun,PENGZhong-tian,LIUYing-xia,etal.DepartmentofInfectiousDiseases,theFirstaffiliatedHospitalofNanhua11University,H
3、unan,Hengyang421001,China【Abstract】ObjectiveToinvestigatetheeffectsofmagnesiumisoglycyrrhizinateontheproliferationandactivityofrathepaticstellatecell.MethodsHSCwereincubatedwithvariousconcentrationsofmagnesiumisoglycyrrhizinatesolution(0mg/ml,0.01mg/ml,0.1mg/ml,1mg/ml,and10mg/ml
4、).WiththeMTTcolorimetricassayandLDH,theeffectsofmaganesiumisoglycyrrhizinateontheproliferationandactivityofrathepaticstellatecellculturedwerestudied,andmorphologicalchangesoftheHSCalsoobserved.ResultsComparedwiththecontrolgroup(0mg/mlgroup),theproliferationofHSCsignificantlydecr
5、easedwhentheconcentrationofmagnesiumisoglycyrrhizinateinmediumreached1.0mg/ml.TherestrainproliferationofHSCpresenteditspeakwhenthemagnesiumisoglycyrrhizinateconcentrationwas10.0mg/ml.DifferentconcentrationsofMagnesiumisoglycyrrhizinatedonotaffectthevitalityonHSCbyLDHmeasurement.
6、ConclusionMagnesiumisoglycyrrhizinatecouldsignificantly11restraintheproliferationofHSCwithincertainconcentrationrange.ButdonotaffectthevitalityonHSCbyLDHmeasurement.【Keywords】Magnesiumisoglycyrrhizinate;HepaticstellatecellsT6;Proliferation;Activity作者單位:421001湖南省衡陽南華大學(xué)附屬第一醫(yī)院感染科(龍
7、素軍彭忠田劉映霞李佳王培);深圳市第三人民醫(yī)院(劉映霞)肝纖維化是多種慢性肝病的共同病理基礎(chǔ)。肝纖維化發(fā)展的各個時期,肝星狀細(xì)胞(hepaticstellatecell,HSC)的活化和細(xì)胞外基質(zhì)合成與降解的失衡是重要病理機(jī)制[1]。甘草酸制劑具有明顯的抗纖維化、抗氧化和抗細(xì)胞凋亡等功效,在臨床上被用于治療肝臟疾病,并取得了顯著的效果。隨著對甘草酸制劑研究的不斷深入,目前己有第四代甘草酸制劑-異甘草酸鎂(Magnesiumisoglycyrrhizinate,MgIG)面世[2]。本文旨在通過從細(xì)胞機(jī)制著手研究和觀察MgIG對HSC增殖和活力的影響,為
8、臨床應(yīng)用提供依據(jù),初步探討MgIG抗纖維化的部分機(jī)制。1材料和方法1.1材料1.1.1細(xì)胞株1
