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1、成都中醫(yī)藥大學(xué)碩士學(xué)位論文學(xué)位論文AStudyonInfluenceofIL.8andTNF.僅ofMouseLungr一●●lI1一o’1。nssneinMOXa-SmogEnVnronments申請(qǐng)學(xué)位級(jí)別:趾論文提交時(shí)間:至Q!至生壘且論文答辯時(shí)間:至Q】至生墨且二。一二年四月【目的】中文摘要探討艾煙對(duì)小鼠肺組織病理、免疫器官及肺部炎癥反應(yīng)的影響,為針灸門診灸法的安全性提供參考?!痉椒ā扛鶕?jù)調(diào)研得知針灸門診最大的艾煙濃度,模擬建立艾煙環(huán)境。以健康昆明小鼠為實(shí)驗(yàn)對(duì)象,隨機(jī)分為空白組、低濃度組、中l(wèi)濃度組和高濃度組,各實(shí)驗(yàn)組每日艾煙煙熏3小時(shí),空白組不進(jìn)行煙
2、熏,實(shí)驗(yàn)時(shí)間為4周,實(shí)驗(yàn)結(jié)束后觀察并分析肺組織切片的病理變化,檢測(cè)并統(tǒng)計(jì)分析胸腺指數(shù)、脾臟指數(shù)及肺組織勻漿IL一8和TNF.a(chǎn)含量的變化。.【結(jié)果】肺組織病理:對(duì)照組支氣管及肺的結(jié)構(gòu)均正常,管壁上皮完整,層次分明,肺內(nèi)血管無(wú)充血和淤血,肺間質(zhì)間隙未見出血、水腫及炎性細(xì)胞浸潤(rùn);低濃度、高濃度組均未見異常;中濃度組有2例出現(xiàn)血管及支氣管周圍水腫,l例出現(xiàn)支氣管管腔出血。胸腺、脾臟指數(shù):各實(shí)驗(yàn)組與空白組比較,各實(shí)驗(yàn)組組間比較均無(wú)統(tǒng)計(jì)學(xué)意義:(P>0.05)肺組織勻漿IL.8和TNF.u含量:各實(shí)驗(yàn)組與空白組比較,各實(shí)驗(yàn)組組間比較均無(wú)統(tǒng)計(jì)學(xué)意義(P>O.05)【結(jié)論】
3、本實(shí)驗(yàn)艾熏條件下,不能使小鼠肺組織出現(xiàn)確切的病理改變及炎癥反應(yīng),且與濃度無(wú)明顯相關(guān)性。提示,臨床上針灸診室使用艾灸,4周內(nèi)不會(huì)對(duì)人體產(chǎn)生確切危害,可以放心使用。關(guān)鍵詞:艾煙,肺,IL一8和TNF.a(chǎn)成都中醫(yī)藥大學(xué)碩士學(xué)位論文[Objective]AbstractWesimulatethedifferentMoxa-smogenvironmenttodiscusswhatimpactofmoxasmoghasonthelungpathology,immuneorgansandthelunginflammatoryreaction,SOastOprovidesec
4、urityfortheuseofmoxibustioninacupunctureclinics.[Methods]Accordingtothemaximumconcentrationofmoxa,whichwehaveinvestigated,wesimulatedifferentMoxa-smogenvironment.ExperimentalsamplesarehealthyKunmingmicewhicharedividedintoseveralexperimentalgroups:Blank,lowconcentration,moderateconce
5、ntrationandhighconcentration.Thesemicearesmokedfor3hourseverydayexcepttheblankgroup.Experimentalperiodis4weeks.Whenexperimentsarefinished,pathologicalchangesoftissuesectionoflungareobservedandanalyzed,andThymusindex,Spleenindex,contentofIL-8andTNF-ainlungtissueshomogenate81"etested,
6、countedandanalyzed.[Results]PathologyofPulmonarytissue:Bronchusandlungtissuesarenormalincontrolgroup.Vesselwallepitheliumsarecompleteandwellarranged,andthereisnocongestionorextravasatedbleedinginbloodvesseloflung.Thereisnobleeding,edemaandinflammatorycellinfiltrationinthegapofpulmon
7、aryinterstitium.Lowconcentrationgroupandhighconcentrationgrouparenormal;moderateconcentrationgrouparenormalexceptthreesamples(twosampleshaveedemaaroundbloodvesselsandbronchus,andonesamplehasbleedinginbronchiallumen.ThymusindexandSpleenindex:Amongexperimentalgroupsandcontrolgroup,its
8、howsnostatisticalsi