肺心清湯對(duì)模型小鼠血清tnf-a及il-8影響的實(shí)驗(yàn)研究

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1、陜西中醫(yī)學(xué)院碩士學(xué)位論文肺心清湯對(duì)模型小鼠血清TNF--a及IL--8影響的實(shí)驗(yàn)研究姓名：許東葉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中西醫(yī)結(jié)合臨床指導(dǎo)教師：韓玲201204中文摘要1111111111II1111111111111111111111111111111Y2115364目的：通過(guò)測(cè)量脂多糖所致模型小鼠血清中TNF．a(chǎn)及IL．8的含量，探討肺必清湯的抗炎作用，進(jìn)一步為臨床治療d,JL急性支氣管炎提供實(shí)驗(yàn)依據(jù)。方法：將昆明種小鼠隨機(jī)分為TNF-a及IL．8兩組(每組各42只)。每組隨機(jī)分為肺必清湯大劑量組、中劑量組、小劑量組，急支糖漿組，模型對(duì)照組

2、，空白對(duì)照組，每組7只。所有老鼠均給予普通飼料，自然采光。肺必清湯大、中、小劑量組分別給予肺必清湯濃度0．439／ml、0．229／ml、O．1lg／ml各0．4ml灌胃給藥，急支糖漿組給予急支糖漿0．4ml灌胃給藥，模型對(duì)照組給予生理鹽水0．4ml灌胃給藥，每天1次，連續(xù)3周，空白對(duì)照組不給予任何處理。3周后各組小鼠均鼻內(nèi)滴注脂多糖50ue]只，每天1次，連續(xù)3天。6小時(shí)后斷頭取血，測(cè)定小鼠血清中TNF．a(chǎn)及IL．8的含量。結(jié)果：模型對(duì)照組即生理鹽水組TNF．a(chǎn),IL．8含量明顯升高，與空白組比較差異非常顯著(p<0．01)。肺必清湯大劑量組

3、TNF．a(chǎn),lL．8含量低于急支糖漿組，兩組比較有差異(p<0．05)。肺必清湯大劑量組n嚇．a(chǎn),IL．8含量明顯低于小劑量組，兩組比較有差異非常顯著(p<0．01)。肺必清湯大劑量組TNF．a(chǎn),IL．8含量低于中劑量組，兩組比較有差異(p

4、ctObjective：Toexploretheroleoffeibiqingsoupbymeasuringthe1ipopolysaccharide—inducedmodelmouseofserumTNF—aandIL一8levels，itwillprovidetheexperimentalbasisforclinicaltreatmentofacutebronchitiSinchildren．Methods：KunmingmicewererandomlydividedintoTNF—aandIL一8groups(n=42)．Eachgrou

5、pwererandomlydividedintofeibiqingsouphighdosegroup。middledosegroupandlowdosegroups，Jizhitangjianggroup，modelcontrolgroupandblankcontrolgroup(n=7)．Allratsweregivenanormaldiet，naturallighting．feibiqingsouphighdosegroup，middledosegroupand10wdosegroupsweregiventhefeibiqingsoupco

6、ncentrationof0．439／ml，0．229／ml，0．1lg／ml，0．4mlgavage，jizhitangjianggroupweretreatedwithjizhitangjiang0．4ml，modelcontrolgroupwasgivennormalsaline0．4mladministeredorally，oncedailyfor3consecutiveweeks，theblankcontrolgroupwerenotgivenanytreatment．a(chǎn)fterthreeweeksMiceineachgroupisi

7、ntranasalinfusionof1ipop01ysaccharide50pg／only1day，3daysinarow．Sixhoursafterdecapitationofblood，determinationofserumTNF-aandIL一8content．Results：modelcontrolgroupTNF—a，IL一8concentrationwassignificantlyincreasedcomparedwiththeblackcontrolgroupasignificantdifference(p

8、biqingsouplargedosegroupTNF—a，IL一8levelslowerthanacutebronchitissyrupgroup，