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1、AbstractdevelopedICSsystemforCLEquantitativedetectioninspikedurinesamplesexhibitedacoefficientofcorrelationat0.97(N=50)withthetraditionalELISAkit.TheperformanceofICSmethodwasfurtherevaluatedbyanalyzing90differentCLEspikedrealurines,theresultsshowedthat96%ofrevovieswereintherangeof70%to130%.The
2、aboveresultsindicatedthattheICSsystemcanbeusedforquantitativeanalysisofclenbuterolinswineurine,andthisnewmethodgreatlyimprovesthedetectionsensitivityofICSandbroadstheapplicationscopeofon-sitedetection.Simultaneously,theT/CratiobasedICSwasalsodevelopedforrapidandquantitativedetectionofclenbuter
3、olresiduesinpork.Fiveofsamplepretreatmentmethodswereevaluated.Theresultsshowedtherecoveryofthepretreatmentmethodinwhichsamplesextractedwith0.02mol/LHClsolutioncontaining2.8%NaClfortwice,wasachievedat76.7%withacoefficientofvariationat7.4%.Thelinearrangeofthenewquantitativemethodwasfrom0.1to1.5n
4、g/gwithadetectionlimitat0.19ng/ginporksamples.Forthespikedsamples,theresultsshowedthattheaveragerecoverieswere60.4±12.8%,70.24±4.2%,75.9±4.9%and71.1±5.0%atCLEconcentrationsof0.5,1,2and3ng/g,respectively.ThenewquantitativesystemforCLEdetectinginspikedporksamplesexhibitedacoefficientofcorrelatio
5、nwith2conventionalELISAmethod(R=0.9136).Besides,immunomagneticbeadsforCLEpurificationwerepreparedbystreptavidin(SA)-biotinsystem.Undertheoptimizedconditions,77.2μgbiotinylatedantibodieswereusedtobindwith1mgmagneticbeadswhichcoatedwith23.9μgstreptavidin.Theoptimaladsorptionandelutionconditionso
6、fimmunomagneticbeadsforCLEpurificationwereasfollows:1gofhomogenizedporkwasextractedwith4mLofHClsolution(0.15mol/L)byvigorousshaking.Aftercentrifugedat16000rpmfor5min,0.25mgmagneticbeadssolutionwasaddedandincubated.Theimmunomagneticbeadswereseparatedwithmagneticshelf,andtheCLEwaselutedfromtheim
7、munomagneticbeadsusing100μLofmethanol.TheperformanceofimmunomagneticbeadswasevaluatedbyusingCLEspikedsamplesatconcentrationof0.25,0.50,1.0,2.0and5.0ng/g,respectively.Theresultsindicatedthattheadsorptionefficiencyofimmunomagneticbeadswer
