活血開(kāi)竅湯對(duì)大鼠腦缺血再灌注損傷no含量與細(xì)胞凋亡的影響

《活血開(kāi)竅湯對(duì)大鼠腦缺血再灌注損傷no含量與細(xì)胞凋亡的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。

1、天津醫(yī)科人學(xué)碩士學(xué)位論文KaiQiaoHuoXue．decoction，adultmaleWistarratswereusedasexperimentalanimals．MaleWistarratswererandomlydividedinto5groups：blankgroup，shamgroup，modelgroupandtherapygroup．Everygrouparebreedindifferentcagesunderthesamecondition．Ratsofthetherapygroupwereintragastricallyadministeredtheapprop

2、riatedosesofChinesemedicineHuoXueKaiQiao-decoction，andthesamedosesofPhysiologicalSalinetotheshamandmodelgroupseachdayfor5daysbeforeischemiainduction，andadministrationwascontinueduntilsacrificeatconclusionoftheexperiment．Thecerebralischemiawasinducedby90minofmiddlecerebralarteryocclusion(MCAO)，w

3、hichwasbasedonthemethoddescribedinitiallybyZea．Longa[1Slwithimprovement．At24hoursand48hoursaftel‘themodelsweremade，werapidlykilltheratsandcompletelytakeoutofthebraintissue，halfofwhicharemadetohomogenatetodetecttheNOcontent．Theotherbrainaretakenafterheartperfusionbyparaformaldehyde，andtobeusedto

4、observetheApoptosisandpathologicalchangebythewayofstaining．WedetectthecontentofNOinthebraintissuefluidsamplesbyusingnitratereductasemethodinstrictaccordancewiththestepsofthekit，observetheapoptosisbyTUNEL，andpathologicalchangebyhematoxylinandeosinstain．ResuItsByexperimentalobservation，pathologic

5、alobservationsindicatethatafter24h，48hthenumberofthedamagednervecelloftheratsthattreatedbyHuoXueKaiQiao．decoctionwaslarge，anddamagedseverely．Whilethedegreeofdamagearesignificantlylighterinthetherapygroup．ItwasdeterminedthatthecontentofNOandtheneuronalapoptosisinthebraintissueofratsinmodelgroupw

6、eresignificantlyincreased(P

7、and48hoursandmitigatethedamagetothebrainIV大津醫(yī)科人學(xué)碩士學(xué)位論文tissuebyreducingthecontentofNO，theexperimentalresultsalsoshowthattheHuoXueKaiQiao-decoctioncaninhibitthedevelopmentofischemiccellsapoptosis，delayorpreventtheneuronaldeathproces