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1、Biomaterials22(2001)511}521Ionicallycrosslinkedalginatehydrogelsassca!oldsfortissueengineering:Part1.Structure,gelationrateandmechanicalpropertiesCatherineK.Kuo!,",PeterX.Ma!,",#,*!DepartmentofBiologicandMaterialsSciences,UniversityofMichigan,1011NorthUniversityAve.,Room2211,AnnArbor,MI4
2、8109-1078,USA"MacromolecularScienceandEngineeringCenter,UniversityofMichigan,AnnArbor,MI48109-1055,USA#DepartmentofBiomedicalEngineering,UniversityofMichigan,AnnArbor,MI48109-2125,USAReceived10March2000;receivedinrevisedform6May2000;accepted8June2000AbstractAlginategelshavebeenusedinboth
3、drugdeliveryandcellencapsulationapplicationsinthebeadformusuallyproducedbydrippingalginatesolutionintoaCaClbath.Themajordisadvantagestothesesystemsarethatthegelationrateishardtocontrol;2theresultingstructureisnotuniform;andmechanicallystrongandcomplex-shaped3-Dstructuresaredi$culttoachie
4、ve.InthisworkcontrolledgelationratewasachievedwithCaCO}GDLandCaSO}CaCO}GDLsystems,andhomogeneousalginategels343wereformulatedassca!oldswithde"neddimensionsfortissueengineeringapplications.Gelationrateincreasedwithincreasingtotalcalciumcontent,increasingproportionofCaSO,increasingtemperat
5、ureanddecreasingalginateconcentration.Mechanical4propertiesofthealginategelswerecontrolledbythecompositionalvariables.Slowergelationsystemsgeneratemoreuniformandmechanicallystrongergelsthanfastergelationsystems.Thecompressivemodulusandstrengthincreasedwithalginateconcentration,totalcalci
6、umcontent,molecularweightandguluronicacid(G)contentofthealginate.MC3T3-E1osteoblasticcellswereuniformlyincorporatedinthealginategelsandculturedinvitro.Theseresultsdemonstratedhowalginategelandgel/cellsystemscouldbeformulatedwithcontrolledstructure,gelationrate,andmechanicalpropertiesfort
7、issueengineeringandotherbiomedicalapplica-tions.(2001ElsevierScienceLtd.Allrightsreserved.Keywords:Alginate;Hydrogel;Ioniccrosslinking;Sca!old;Tissueengineering;Polymer1.Introductionofb-D-mannuronicacid(M)anda-L-guluronicacid(G)[16,17].Becauseoftheirbiocompatibility,abund
