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《乳化異氟醚預(yù)處理對(duì)大鼠局灶性腦缺血再灌注損傷與p-akt活性的影響》由會(huì)員上傳分享，免費(fèi)在線(xiàn)閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、徐州醫(yī)學(xué)院碩士學(xué)位論文Effectofemulsifiedisofluranepreconditioningonfocal_l‘●‘』■●l●·’1cereDraliSCnemm-rel3ertusionnn]UrvISmeoiateoDVP13l饑～ktpathway—It／AbstractobjectiveToinvestigatetheprotectiveeffectofE1preconditioningonfocalcerebralischemia-reperfusion(I瓜)injuryinrats；toapproa
2、chthemechanismofcerebralprotectionbydetectingthechangeofphosphorylationofserine
3、threonineldnaseQ—Akt)activity．PARTlMethodsFocalcerebralI／RmodelinratsWaSmadeby(MCAO)．FortyeightadultmaleSprague-Dawley(SD)ratswererandomlydividedintosixgroups(n尋8)：SHAMgroup，F(xiàn)Rgroup，lowdo
4、seofElpreconditioninggroup(El-Lgroup)，mediumdoseofEIpreconditioninggroup(El-Mgroup)，highdoseofEIpreconditioninggroup(EI-Hgroup)，intralipid(vehicleforisoflurane)group(ILgroup)．SHAMgroupwasadministeredsaline10．5ml／kg,24hlaterseparatebloodvesselsonly，anddon’tinsertthesu
5、ture．I／Rgroup，El—Lgroup，E1一Mgroup，E1-Hgroupwereintraperitoneallyadministeredsaline10．5ml／kg,El3．5ml&酣NS7ml／kg，El7ml／kg+NS3．5ml／kg,E110．5ml／kgand30％intralipid10．5ml／kgrespectively,and24hlatersubjectedtoMCAO．Rectaltemperature，heartrate，andrespiratoryratewererecored10mi
6、nutesbeforebrainischemiaand10minutesafterI／R．After24hofreperfusion,neurologicaldeficitScOreSwereassessed．CerebralinfarctionvolumeWasinvestigatedineachgroup．Results1)After10minutescerebralI／R，rectaltemperatureandheartrateofratsincreased，andrespiratoryratedecreased(P<0
7、．05)．ThisfullydemonstratesthatthemodelWassuccessful．InadditiontoSHAMgroup，thereisnosignificantdifferencebetweenothergroupsat10minutesafterI／Rinbodytemperature，heartrate，respiratoryrate(P>0．05)．2)ComparedwithSHAMgroup，neurologicaldeficitandcerebralinfarctvolumeincreas
8、edinothergroupsC尸≮O．01)；ComparedwimI／Rgroup，neurologicaldeficitandcerebralinfarctvolumereducedinElpreconditioninggroups(．尸<0．05)；ComparedinElpreconditioninggroups，neurologicaldefcitandcerebralinfarctvolume4●徐州醫(yī)學(xué)院碩士學(xué)位論文reduceddose-dependently(P<0．05)．Continuepart2witl
9、l10．5ml／kgEIpreconditioning．PART2MethodsAnotherf．o啊eightadultmaleSprague-Dawley(SD)ratswererandomlydividedintosixgroups(n=8)：SHAMgr
