針刺血清對(duì)體外培養(yǎng)神經(jīng)細(xì)胞內(nèi)鈣離子濃度的影響

《針刺血清對(duì)體外培養(yǎng)神經(jīng)細(xì)胞內(nèi)鈣離子濃度的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。

1、中西醫(yī)結(jié)合學(xué)報(bào)2004年11月第2卷第6期JChinIntegrMed,Nov2004,Vol.2,No.6·453·針刺血清對(duì)體外培養(yǎng)神經(jīng)細(xì)胞內(nèi)鈣離子濃度的影響李瑞午,張靜齡,郭瑩,李翠紅(中國(guó)中醫(yī)研究院針灸研究所,北京100700)2+[摘要]目的:通過(guò)觀察針刺大鼠經(jīng)穴后收集的血清對(duì)體外培養(yǎng)的神經(jīng)細(xì)胞內(nèi)Ca濃度的影響,為探討針灸作用的機(jī)制中是否存在著體液因素提供直接的證據(jù)。方法:取新生大鼠大腦皮層細(xì)胞進(jìn)行原代培養(yǎng)。7～10d后應(yīng)用熒光分子探針Fluo-3AM染色,激光共焦掃描顯微鏡動(dòng)態(tài)掃描觀察加

2、入正常大鼠血清和電針刺激百會(huì)、足三里、曲池、三陰交2周后的血清對(duì)神經(jīng)細(xì)胞2+2+2+內(nèi)Ca濃度的影響。結(jié)果:加入正常血清后,細(xì)胞內(nèi)的Ca濃度會(huì)有一定的升高,經(jīng)過(guò)一段時(shí)間后升高的Ca濃度會(huì)趨向平2+穩(wěn)。而再加入針刺血清后,在一定程度上可以降低神經(jīng)細(xì)胞Ca的濃度。結(jié)論:針刺腧穴后,除了神經(jīng)的調(diào)節(jié)機(jī)制外,針刺血2+清中存在某種活性物質(zhì),可以明顯地使神經(jīng)細(xì)胞內(nèi)Ca濃度降低,為針灸體液因素的存在提供了直接的證據(jù)。[關(guān)鍵詞]針刺療法;血清學(xué);神經(jīng)細(xì)胞;鈣離子[中圖分類(lèi)號(hào)]R245[文獻(xiàn)標(biāo)識(shí)碼]A[文章編號(hào)]16

3、72-1977(2004)06-0453-032+EffectofacupunctureserumonintracellularCaconcentrationinculturedneuronsLIRui-Wu,ZHANGJin-Ling,GUOYing,LICui-Hong(InstituteofAcupuncture-Moxibustion,ChinaAcademyofTraditionalChineseMedicine,Beijing100700,China)ABSTRACTObjective:

4、Tostudythehumoralfactorofacupunctureinimprovingfunctionalactivitiesoftheorganism.Methods:Neuronsofthecerebralcortexfromthenewbornratsweretakentobeculturedinastrictlycontrolledmedium.2+2+Seventotendayslater,fluorescein-molecularprobeFluo-3AMwasusedforst

5、ainingintracellularCa.Caconcentrationanditschangesafterapplicationofacupunctureserumweremeasuredbyusingalaserconfocalmicroscope.Normalserumandacupunctureserumwerederivedfromthenormalcontrolratsandtheratsundergoingelectro-acupunctureof“Baihui(GV20)”,“Zu

6、sanli(ST36)”,“Quchi(LI11)”and“Sanyinjiao(SP6)”for2weeks.Results:Afterapplicationofnormalserum,theintra-2+cellularCaconcentrationincreasedstrikinglyimmediatelyatthebeginning,andgraduallygotstableafterwards.Whenacupuncture2+serumwasaddedtotheculturefluid

7、,Caconcentrationloweredsignificantly.Conclusion:Theseresultssuggestthatbioactivesub-2+stancesinserumcollectedfromratsundergoingacupuncturecanaffecttheintracellularCaconcentrationofthecerebralneurons,andthehumoralfactormayparticipateintheeffectofacupunc

8、tureinregulatingfunctionalactivitiesoftheorganism.2+KEYWORDSacupuncture;serology;nervecells;CaJChinIntegrMed,2004,2(6):453-455①針灸調(diào)整和針刺鎮(zhèn)痛機(jī)制的研究一直是醫(yī)學(xué)界針刺作用的機(jī)制提供了細(xì)胞生物學(xué)和血清學(xué)的依據(jù)。研究的熱門(mén)課題。以往的研究多從神經(jīng)體液方面入1材料與方法手,其中又以神經(jīng)機(jī)制的探討為主。體液因素的研究相對(duì)較少,尤其缺少直接的證據(jù)。