供精人工授精不同結(jié)局精子蛋白質(zhì)組成差異的研究

供精人工授精不同結(jié)局精子蛋白質(zhì)組成差異的研究

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時(shí)間:2019-03-04

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1、碩士學(xué)位論文學(xué)校代碼:10023學(xué)號(hào):$2010020008供精人工授精不同結(jié)局精子蛋白質(zhì)組成差異的研究Proteomeanalysisofspermindifferentoutcomes^o,'‘‘1·1orartltlClaJ-1nsemlnatlonbydonor所院:國(guó)家人口計(jì)生委科學(xué)技術(shù)研究所姓名:李志廣指導(dǎo)教師:谷翊群研究員導(dǎo)師小組:于和鳴盧文紅辛玲學(xué)科專(zhuān)業(yè):外科學(xué)研究方向:泌尿男科完成日期:2013年4月20日北京協(xié)和醫(yī)學(xué)院碩士研究生學(xué)位論文唧幽舢舢舢刪刪舢舢刪Y2340232目錄中文摘要································

2、···.·..···...................1英文摘要··········--·····················-·····-·····················2—1上.—j一日H舌”一“”一”一””‘。?!?。。··。。-····--··-·······················3第一部分:供精人工授精不同結(jié)局精子蛋白質(zhì)組成差異實(shí)驗(yàn)材料·························---·-·········0Q411················8實(shí)驗(yàn)方法·······-·························-

3、·······················10實(shí)驗(yàn)結(jié)果·······-·················································14實(shí)驗(yàn)討論······-··················-············-········-·········16實(shí)驗(yàn)小結(jié)·································-··················-····18參考文獻(xiàn)·-····················--··-·························-····19第二部分:供精人工

4、授精不同結(jié)局精子差異蛋白質(zhì)譜鑒定實(shí)驗(yàn)材料-···················-············-············-··········21實(shí)驗(yàn)方法················································-·-······21實(shí)驗(yàn)結(jié)果··········-·-·---·································-·-····23實(shí)驗(yàn)討論··········································-·····-········25實(shí)驗(yàn)小結(jié)··············

5、············--··-··························29參考文獻(xiàn)·-·······················································30第三部分:精液凝固蛋白.1和谷胱甘肽硫轉(zhuǎn)移酶Mu3蛋白在供精人工授精不同結(jié)局精子蛋白中的表達(dá)差異實(shí)驗(yàn)材料·············-·-··············-··························33實(shí)驗(yàn)方法······································-·············-····35實(shí)驗(yàn)結(jié)果

6、················-················-·······················37實(shí)驗(yàn)討論·························································37實(shí)驗(yàn)小結(jié)····-·············---······-······-··················-···39參考文獻(xiàn)·························································40結(jié)論············-·········--············

7、-······-·················-···42文獻(xiàn)綜述··-·····-····················································43英文縮略詞表(Abbreviation)······-···············-···················52致謝···············-······································-······53個(gè)人簡(jiǎn)歷·········-·······················-···--··············

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