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1、徐州醫(yī)學(xué)院碩士學(xué)位論文和NR1和PCNA免疫熒光雙標(biāo)細(xì)胞的細(xì)胞;P1d到P7d時(shí),NR1和GFAP的區(qū)域共存程度非常低;到了P14d以后,二者在該區(qū)區(qū)域共存程度增加。6.給予NMDA受體激動(dòng)劑NMDA(2mg/kg/d),在P7d時(shí),SVZ細(xì)胞密度明顯增加(P<0.05);P14d時(shí)PCNA+細(xì)胞密度明顯增加。分別給予MK-801(10mg/kg)和Ro25-6981(40mg/kg),P7d時(shí)PCNA陽性細(xì)胞密度均顯著降低(P<0.05),從P14d以后逐漸升高。到P28d時(shí)明顯高于正常(P<0.05),其中Ro25-6981組升高的更明顯。結(jié)論大鼠生后早期
2、SVZ中的細(xì)胞表達(dá)有NMDA受體亞單位NR1、NR2A、NR2B,隨著年齡的增長(zhǎng),不同的亞單位表達(dá)呈現(xiàn)不同的變化趨勢(shì),其中部分細(xì)胞具有增殖特性。激活NMDA受體后對(duì)SVZ中細(xì)胞的增殖有正性調(diào)節(jié)作用,提示SVZ區(qū)內(nèi)的NMDA受體具有功能活性,可以參與調(diào)節(jié)生后早期SVZ區(qū)的神經(jīng)發(fā)生。關(guān)鍵詞NMDA受體;SVZ;細(xì)胞增殖;PCNA項(xiàng)目資助來源:1.江蘇省普通高校研究生科研創(chuàng)新計(jì)劃項(xiàng)目,200807。2.江蘇省麻醉學(xué)重點(diǎn)實(shí)驗(yàn)室開放課題(KJS07005)3.江蘇省高校自然科學(xué)基礎(chǔ)研究計(jì)劃項(xiàng)目(07KJB310119)6徐州醫(yī)學(xué)院碩士學(xué)位論文NMDAreceptorsu
3、bunitsinSVZanditsroleinneurogenesisofearlypostnatalrat’sbrainABSTRACTObjectiveStudythenormalexpressionofNMDAreceptorsubunitNR1,NR2A,
NR2Bandidentifythecelltypeofreceptorexistence,aswellasitsroleincell
proliferationinthesubventricularzone(SVZ)oftherat’sbrainduringtheearly
postnatalpe
4、riod.Methods6.Theearlypostnataldifferentagepointrats(postnatalday1,P1d;P3d;P7d;P14d;P21d;P28d)wereusedtotesttheexpressionchangesoftheNMDAreceptor
subunitNR1,NR2AandNR2BintheSVZ,aswellastheastrocytes(GFAP+),
matureneurons(NeuN+)andproliferationcells(PCNA+)atthesamezoneby
immunohistoc
5、hemicalmathedandHEstaining.7.Theinmmunofluorescencedouble-labeledmathedwasusetoabservethe
co-expressionofthereceptorsubunitNR1andNestinorNR1andGFAPaswellas
NR1andPCNAintheSVZ.8.RatswereinjectedintraperitoneallyofNMDAreceptoragonistNMDA(2mg/kg/d),selectivenon-competitiveNMDAreceptora
6、ntagonistMK-801(10mg/kg)and
NR2BantagonistRo25-6981(40mg/kg)respectivelyattheageP3dtoobservethe
celldensitychangesoftheproliferationcellsintheSVZofP7d,P14dandP28d’
rat’sbrainbyimmunohistochemicalmathedandHEstaining.Results1.ThetotalcellcountanddensityoftheSVZdecreasedwithageduringth
7、eearlypostnatalperiod.Atthepostnatal1day(P1d),thecelldensitywas269.50±
9.60/0.01mm2;P3d:265.40±8.29/0.01mm2;P7d:167.40±27.10/0.01mm2;
P14d:120.00±16.98/0.01mm2;P21d:72.50±7.78/0.01mm2;P28d;64.00±
8.46/0.01mm2.2.TheNMDAreceptorsubunitNR1,NR2AandNR2BexpressedintheSVZ’scellsoftheratbra
8、inineachtestedpostn