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《乳腺癌患者cd4^＋cd25^highccr6^＋調(diào)節(jié)性t細(xì)胞的變化》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫(kù)。
1、四川大學(xué)學(xué)醫(yī)學(xué)版2。10JSichuanUniv(MedSeiEdi)。；41(3)：’4l5—419乳腺癌患者CD4+CD25ghCCR6+調(diào)節(jié)性T細(xì)胞的變化徐林，周涯。，肖代敏。，覃明，羅軍敏，湯賢英1．貴州省遵義醫(yī)學(xué)院免疫學(xué)教研室(遵義563003)；2．貴州省遵義醫(yī)學(xué)院醫(yī)學(xué)物理學(xué)教研室(遵義563003)；3．貴州省遵義醫(yī)學(xué)院附屬醫(yī)院檢驗(yàn)科(遵義563003)【摘要】目的檢測(cè)臨床乳腺癌患者腫瘤浸潤(rùn)淋巴細(xì)胞(TILs)和外周血單個(gè)核細(xì)胞(PBMCs)中CD4CD25h~CCR6調(diào)節(jié)性T細(xì)胞的變化，并探討其意義。方法分離22例乳腺癌患者PBMCs和TILs，用流式細(xì)胞儀檢測(cè)CD4C
2、D25~CCR6調(diào)節(jié)性T細(xì)胞的比例，同時(shí)檢測(cè)其Foxp3的表達(dá)；進(jìn)一步用流式細(xì)胞儀檢測(cè)CD4CD25~CCR6調(diào)節(jié)性T細(xì)胞記憶分子CD45R0、CD44和CD62L的表達(dá)水平；用。H_TdR增殖實(shí)驗(yàn)觀察CD4CD25CCR6調(diào)節(jié)性T細(xì)胞對(duì)CD4CD25一T細(xì)胞增殖的抑制作用。結(jié)果乳腺癌患者PBMCs和TILs中CD4CD25~CCR6調(diào)節(jié)性T細(xì)胞的比例明顯增加，并在腫瘤局部存在顯著的富集；CD4CD25“CCR6調(diào)節(jié)性T細(xì)胞高表達(dá)Foxp3，在體外能有效抑制CD4CD25～T細(xì)胞的增殖，并高表達(dá)記憶分子CD45RO、CD44，低表達(dá)CD62L，顯示了效應(yīng)／記憶樣表型。結(jié)論CD4CD25
3、highCCR6調(diào)節(jié)性T細(xì)胞在乳腺癌患者腫瘤局部有明顯富集，這可能是腫瘤長(zhǎng)期免疫逃逸的重要原因?！娟P(guān)鍵詞】CD4CD25~,CCR6調(diào)節(jié)性T細(xì)胞乳腺癌腫瘤浸潤(rùn)淋巴細(xì)胞【中圖分類號(hào)】R734．2TheChangeofCD4+CD25咖CCR6+RegulatoryTCellsinBreastCancerPatientsXULin，ZHOUYa。，XIAODai-min。，QINMing，LUOJun—min，TANGXian—ying．1．DepartmentofImmunology，ZunyiMedicalCollege，Zunyi563003，China；2．Departmentof
4、MedicalPhysics，ZunyiMedicalCollege，Zunyi563003，China；3．DepartmentofClinicalLaboratory，ZunyiMedicalCollege，Zunyi563003，China[Abstract]ObjectiveTodetecttheproportionofCD4CD25highCCR6regulatoryTcellsinperipheralbloodmononuclearcellsandtumorinfiltrationlymphocytesfrombreastcancerpatientsandexploreit
5、ssignificance．MethodsLymphocytesisolatedfrombloodandtumormassofbreastcancerpatientswereanalyzedfortheproportionofCD4CD25~CCR6regulatoryTcellsbyflowcytometry．TheexpressionofFoxp3onCD4CD25h*CCR6regulatoryTcells，aswellasCD45R0，CD44andCD62L，werealsoanalyzed．TheeffectsofCD4CD25~CCR6regulatoryTcellson
6、theproliferationofCD4CD25一Tcellsalsoweredetectedby。H—incorporationassay．ResultsComparedtothecontrol，increasedproportionofCD4CD25~CCR6regulatoryTcellswasobservedinPBMCsandTILsfrombreastcancerpatients．Moreover，CD4CD25big“CCR6regulatoryTcells，expressinghighlevelofFoxp3，displayedeffectormemoryphenot
7、ypedeterminedbyhighlevelexpressionofCD45RO，CD44andlowlevelofCD62L．Inaddition，CD4CD258hCCR6regulatoryTcellscouldinhibittheproliferationofCD4CD25一Tcellsinvitro．ConclusionTheenrichmentofCD4CD25CCR6regulatoryTcellsintumormassinb
