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1、WhatisMicroarray￠Apowerfultechnologyforbiologicalexplorationwhichenablestosimultaneouslymeasuretheenablestosimultaneouslymeasurethelevelofactivityofthousandsgenes.￠TheamountofmRNAforeachgeneinagivenTheamountofmRNAforeachgeneinagivensample(orapairofsamples)ismeasured.￠Microarraysare:￠Parall
2、el￠HighHigh--throughputthroughput￠Largearge--scascallle￠GenomicscaleMostknowncommercialmicroarrayplatformsAppliedAgilentAffymetrixNembGeneIlluminaBiosystemInkjetContactMirrorPhotolithograBeads-linkedsynthesisspottiiingphlhotoliithhographyoligolibraryphy60mers25mers60mers24mers50mers￠Commer
3、cialmicroarraysgiveingeneralmorereproducibleresultsduetothhhihQCehighQC,whihihchcanbeachievedonlyatindustriallevel.RobotRobotpinspottingRobotpinspottingAggjilentinkjettechnologygy￠Agilklentusesinkjettechhnollogytoprintollligosontoglassslides.￠ThenonThenon-contactinkjettechnologyproducesmic
4、roarrayscontactinkjettechnologyproducesmicroarrayswithmoreuniformandconsistentfeatures.￠Theinkjjpetprocessdoesnotintroducedefectsasaresultofsurfacetensioninteractionswiththemicroarraysurface.￠NonNon--contactinkjetprocessprovidessubstantialcontactinkjetprocessprovidessubstantialitimprovemen
5、toverpiinspottittingwithithrespecttttoconsiisttencyandspotuniformity.Probedesign￠Proprietaryalgorithmsarealgorithmsareusedtodesigntheprobes.￠Designofconsensustargetsequencesisbasedsequencesisbasedontheintegrationofpublicdatadidbdiffderivedbydifferenttresources.￠ProbesareProbesareexperiment
6、allyvalidated.Affyyymetrixmicroarrays￠a.k.agenechip.￠Insteadofputtingentiregenesonanarray,putputsetsofDNA25putsetsofDNA25-mers(oligonucleotides)mers(oligonucleotides).￠ProducedusingaphotolithographyprocessProducedusingaphotolithography,processsimilartothatusedtomakesemiconductorchips.￠mRNA
7、samplesareprocessedseparatelymRNAsamplesareprocessedseparatelyinsteadofinpairs(singlechanneltechnology)AffyygpmetrixgeneChips￠Various25mersareputonthechiptointerrogateasinggglegene.￠Additionallyaslightvariant(thatdiffersonlyatthe13thbase)isputnexttoitbase)ispu
