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《獨(dú)角蓮塊莖提取液抗腫瘤活性的初步研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、摘要獨(dú)角蓮作為一種名貴中草藥，具有較高的醫(yī)療價(jià)值和防病保健功能，將其塊莖提取液應(yīng)用于抗腫瘤研究，并探討其相關(guān)機(jī)制。本文以東北產(chǎn)獨(dú)角蓮塊莖為原料，以蒸餾水做提取溶劑．以溶劑量是藥材的10倍為原則進(jìn)行加量提取，提取方式采用超聲波，時(shí)間為20min，間隔lmin后再重復(fù)一次。對(duì)真空干燥后得到獨(dú)角蓮塊莖提取物的活性分析表明，對(duì)酪氨酸酶的相對(duì)抑制率51．21％，達(dá)到最佳效果。MTT實(shí)驗(yàn)結(jié)果表明，獨(dú)角蓮塊莖提取液對(duì)體外Hela的增殖有一定的抑制作用：對(duì)Hcla的抑制率為65．08％。細(xì)胞凋亡實(shí)驗(yàn)和細(xì)胞周期流
2、式細(xì)胞儀檢測(cè)表明，獨(dú)角蓮塊莖提取液能引起凋亡細(xì)胞的增加，并成劑量依賴性，獨(dú)角蓮塊莖提取液作用72h后的凋亡率最高，為24．5％。Westernblot檢測(cè)HeLa細(xì)胞經(jīng)濃度遞增的塊莖提取液處理48h后，caspasc3蛋白表達(dá)逐漸增強(qiáng)。獨(dú)角蓮塊莖提取液對(duì)癌細(xì)胞的生長(zhǎng)抑制呈現(xiàn)出良好的量效關(guān)系。對(duì)接種$180細(xì)胞株形成實(shí)體瘤的小鼠用獨(dú)角蓮塊莖提取液灌胃表明，獨(dú)角蓮有顯著的抗腫瘤活性，抑瘤率為31．77％。對(duì)腹腔接種EAC細(xì)胞株形成腹水癌的昆明種小鼠用獨(dú)角蓮提取液灌胃表明獨(dú)角蓮能延長(zhǎng)腹水癌小鼠生存期，
3、生命延長(zhǎng)率為43．26％；能改善腹水癌小鼠的生存狀態(tài)。結(jié)論顯示獨(dú)角蓮塊莖提取液有顯著的抗腫瘤作用。關(guān)鍵詞：獨(dú)角蓮塊莖提取液抑制癌細(xì)胞抑瘤作用ABSTRACrTyphoniumlotusiSakindofChinesetraditionalmedicine．致hashighmedicalvalueandfunctionofpreventsdisease．Theextractionoftyphoniumlotustuberwasusedtostudyjtsantitumoractivityanddi
4、scusseditsrelatedmechanism．UsethetuberofNortheasttyphoniumlotusasmaterial．with10timesofdistilledwaterasextractionsolvent，extractionmethodsisusingultrasonic，thetimeis20rain，onerepeatafterlmininterval。theextractionoftyphoniumlotustuberisobtainedafterva
5、cuumdrying．It’Sthebestrelativeinhibitionrateontyrosinaseis51．21％．TheresultsofMTTshowthattheextractionoftyphoniumlotustuberhassomecertaininhibitionontheproliferationofHela，andinhibitionrateis65．08％．Apoptosisandflowcytometryanalysisshowthattheextractio
6、noftyphoniumlotustubercancausetheincreaseofapoptoticcells．a(chǎn)ndthisphenomenonisdose—dependent．T．IleapoptosisrateisthehigIlestaftertreatedwith72hbytheextractionoftyphoniumlotustuber,reachto24．4％．W色sternblotdetectHelacellswastreated48hbyaseriesofconcentr
7、ationofextraction，theexpressionofcaspase3wasincreasedgradually．Itappearedgooddose·effectrelationshipabouttheextractionoftyphoniumlotustuberoncancereellinhibition．TheextractionoftyphoniumlotustuberonthemicewithsolidtumorbyinoculaS180celllineshowthatan
8、ti-tumoractivityoftyphoniumlotusissignificant，theinhibitionratereachto31．77％．ExperimentofextractionoftyphoniumlotustuberthatgavagedtoKunmingmicewithascitescarcinomabyinoculaEACcdllinesshowsthat：theextractioncanextendsurvivaltime。theextendedratereacht
