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《水稻窄葉突變體nal10的鑒定與基因精細(xì)定位》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、中國水稻科學(xué)(ChinJRiceSci),２０１５,２９(６):５８７－５９４http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０６．００４５８７水稻窄葉突變體nal１０的鑒定與基因精細(xì)定位方云霞１,２,３,＃朱麗２,＃潘江杰４余海平２薛大偉４饒玉春２王小虎２石珍源２張棟４祝陽舟２董國軍２曾大力２張光恒２郭龍彪２胡江２,?錢前２,?(１浙江大學(xué)農(nóng)業(yè)與生物技術(shù)學(xué)院,杭州３１００５８;２中國水稻研究所水稻生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室,杭州３１０００６;３江西省農(nóng)業(yè)科學(xué)院
2、水稻研究所,南昌３３０２００;４杭州師范大學(xué)生命與環(huán)境學(xué)院,杭州３１００３６;＃共同第一作者;?通訊聯(lián)系人,EＧmail:qianqian１８８＠hotmail．com;hujiang５８８＠１６３．com)IdentificationandFineMappingofaNarrowLeafMutantnal１０inRiceFANGYunＧxia１,２,３,＃,ZHULi１,＃,PANJiangＧjie４,YUHaiＧping２,XUEDaＧwei４,RAOYuＧchun２,WANGXiaoＧhu２,SHIZhenＧy
3、uan２,ZHANGDong４,ZHUyangＧzhou２,DONGGuoＧjun２,ZENGDaＧli２,ZHANGGuangＧheng２,GUOLongＧbiao２,HUJiang２,?,QIANQian２,?(１CollegeofAgricultureandBiotechnology,ZhejiangUniversity,Hangzhou３１００５８,China;２StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzho
4、u３１０００６,China;３RiceResearchInstitute,JiangxiAcademyofAgriculturalSciences,Nanchang３３０２００,China;４CollegeofLifeandEnvironmentalSciences,HangzhouNormalUniversity,Hangzhou３１００３６,China;＃Theseauthorscontributedequallytothiswork;?Correspondingauthors,EＧmail:qianqian１８
5、８＠hotmailc．om;hujiang５８８＠１６３c．om)FANGYunxia,ZHULi,PANJiangjie,etal．Identificationandfinemappingofanarrowleafmutantnal１０inrice．ChinJRiceSci,２０１５,２９(６):５８７Ｇ５９４．Abstract:Anovelricemutantnarrowleaf１０(nal１０)wasidentifiedfromanEMS(ethylmethanesulphonate)mutagenizedpo
6、pulationofWuyunjing７．Themutantwascharacterizedbynarrowleavesandsmallerarchitecturephenotypeattheseedlingstage,anddwarf,narrowleaves,deformedflowersandmalesterilityatthereproductionstage．CytoＧanatomicalanalysisindicatedthatthemutanthadfewerlargeandsmallveinscomp
7、aredwiththewildＧtype,whichmayaccountforthenarrowleafphenotype．Thegeneticanalysisindicatedthenarrowleaftraitwascontrolledbyarecessivenucleargene．UsingF２populationderivedfromthecrossofnal１０andTaizhongbendi１,threecloselylinkedSSRmarkersRM１２８７,RM５６２andRM５６３８onchrom
8、osome１wereobtainedbythebulkedsegregantanalysismethod．Withthedevelopednewmarkers,thegenewasfinallyfineＧmappedtoanintervalof５５．２kbbetweentheSTSmarkersPK８３andPK７８,whereatotalof
