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1、中國(guó)水稻科學(xué)(ChinJRiceSci),2015,29(6):595-600http://www.ricesci.cnDOI:10.3969/ji.ssn.1001G7216.2015.06.005595水稻窄卷葉突變體Nrl3(t)的基因定位張小惠1,2,3,#秦亞芝1,2,#張迎信2占小登2張振華2沈希宏2程式華2曹立勇2,?吳先軍1,?(1四川農(nóng)業(yè)大學(xué)水稻研究所/西南作物基因資源與遺傳改良教育部重點(diǎn)實(shí)驗(yàn)室,成都611130;2中國(guó)水稻研究所國(guó)家水稻改良中心,杭州310006;3浙江國(guó)稻高科技種業(yè)有限公司杭州
2、311401;#共同第一作者;?通訊聯(lián)系人,EGmail:wuxjsau@126.com;caolycgf@m(xù)ail.hz.zj.cn)GeneMappingofaNarrowandRolledLeafMutantNrl3(t)inRiceZHANGXiaoGhui1,2,3,#,QINYaGzhi1,2,#,ZHANGYingGxin2,ZHANXiaoGdeng2,ZHANGZhenGhua2,SHENXiGhong2,CHENGShiGhua2,CAOLiGyong2,?,WUXianGjun1,?(1Ric
3、eResearchInstitute/KeyLaboratoryofSouthwestCropGeneticResourcesandImprovement,MinistryofEducation,SichuanAgriculturalUniversity,Chengdu611130,China;2NationalCenterforRiceImprovement,ChinaNationalRiceResearchInstitute,Hangzhou,310006,China;3ZhejiangNationalRice
4、HighGTechPlantsIndustryLimitedCompany,Hangzhou311401,China;#Theseauthorscontributedequallytothiswork;?Correspondingauthors,EGmail:wuxjsau@126c.om;caolycgf@m(xù)ail.hz.zjc.n)ZHANGXiaohui,QINYazhi,ZHANGYingxin,etal.GenemappingofanarrowandrolledleafmutantNrl3(t)inric
5、e.ChinJRiceSci,2015,29(6):595G600.Abstract:Nrl3(t)wasanovelnarrowandrolledleafmutantfromtheindicarestorerlineZhonghui8015afterexposure60toCoGγray.Nrl3(t)exhibitedsignificantadaxiallyGrolledleaves,reducedplantheight,shortenedpaniclelength,reducedgrainlength,gra
6、inwidthandthousandGgrainweight,aswellastheseedGsettingrate.Cytologicalanalysisshowedthattherolledleafphenotypewasduetothereducednumberofvascularbundleandthechangedsizeofbulliformcells.InaninitialmappingwithF2individualsderivedfromacrossbetweenNrl3(t)andjaponic
7、a02428,thenarrowandrolledleafgeneNrl3(t)waseventuallymappedtoa70.3kbregiononthelongarmofchromosome2betweentwonewlydevelopedsequencetaggedsitemarkersC9andC10E.TheseresultswouldlayagoodfoundationformolecularcloningandfunctionalanalysisofNrl3(t).Keywords:rice;nar
8、rowandrolledleaf;gene;finemapping張小惠,秦亞芝,張迎信,等.水稻窄卷葉突變體Nrl3(t)的基因定位.中國(guó)水稻科學(xué),2015,29(6):595G600.摘要:利用60CoGγ射線輻射誘變秈稻恢復(fù)系中恢8015,獲得了一份窄卷葉突變體Nrl3(t).與野生型中恢8015相比,突變體葉片顯著變窄內(nèi)卷、株高降低、穗長(zhǎng)變短、結(jié)實(shí)率