資源描述：
《轉(zhuǎn)Cry30Fa1基因抗褐飛虱水稻的獲得及鑒定》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、中國水稻科學(xué)(ChinJRiceSci),２０１６,３０(３):２５６－２６４http://www．ricesci．cn２５６DOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１９０轉(zhuǎn)Cry３０Fa１基因抗褐飛虱水稻的獲得及鑒定王海鵬黃曉西梁越洋朱軍張翠霞王秀梅貢常委鄭愛萍鄧其明李雙成王玲霞李平王世全?(四川農(nóng)業(yè)大學(xué)水稻研究所,四川溫江６１１１３０;?通訊聯(lián)系人,EＧmail:sqwangscau＠１６３．com)DevelopmentandIdentificationofInsectResistantTransgenicRicewithCry３０
2、Fa１GeneWANGHaiＧpeng,HUANGXiaoＧxi,LIANGYueＧyang,ZHUJun,ZHANGCuiＧxia,WANGXiuＧmei,GONGChangＧwei,ZHENGAiＧping,DENGQiＧming,LIShuangＧcheng,WANGLingＧxia,LIPing,WANGShiＧquan?(RiceResearchInstitute,SichuanAgriculturalUniversity,Wenjiang６１１１３０,China;?Correspondingauthor,EＧmail:sqwangscau＠１６３c
3、．om)WANGHaipeng,HUANGXiaoxi,LIANGYueyang,etal．DevelopmentandidentificationofinsectresistanttransgenicricewithCry３０Fa１gene．ChinJRiceSci,２０１６,３０(３):２５６Ｇ２６４．Abstract:ModifiedCry３０Fa１,aBacillusthuringiensis(Bt)geneencodingahighＧefficiencyinsecticidalproteintothebrownplanthopper(BPH),was
4、introducedintoShuhui８１８(R８１８)byAgrobacteriumＧmediatedgenetictransformationand４６transgenelineswereobtained．WeconfirmedthevalidtransformationbyRTＧqRCRandWesternblotattranscriptionallevelandtranslationallevel,andfixedthestableexpressionCry３０Fa１inhomozygosis．Theclassicalpedigreebreeding
5、methodswerealsousedtoselecttheimportantagronomictraitsintransgeniclines．Furthermore,weevaluatedtheresistancetoBPHofdifferentlinesingrowthchamberandfield,andfoundthattheR８１８ＧCry３０FaldisplayedhigherresistancethanuntransformedR８１８inseedlingstage．WealsoobservedthereducedpopulationofBPHf
6、eedingthetransgeniclines,suggestingthatthetransformedCry３０Fa１enhancedresistibilitytoBPHinrice．WecultivatednewBTproteintransgenicricerestorerlineR８１８ＧCry３０Fal,whichmayprovidenewBPHresistancematerialtothreelinehybridricebreedingandenrichBPHresistancegermplasmresourcesinrice．Keywords:b
7、rownplanthopper;AgrobacteriumＧmediatedmethod;insectresistance;Cry３０Fa１王海鵬,黃曉西,梁越洋,等．轉(zhuǎn)Cry３０Fa１基因抗褐飛虱水稻的獲得及鑒定．中國水稻科學(xué),２０１６,３０(３):２５６Ｇ２６４．摘要:將編碼高效殺褐飛虱蛋白的蘇云金芽胞桿菌基因Cry３０Fa１密碼子改造后,通過農(nóng)桿菌介導(dǎo)法轉(zhuǎn)入蜀恢８１８(R８１８),并最終獲得４６個(gè)轉(zhuǎn)基因植株.通過定量PCR及WesternBlot鑒定了Cry３０Fa１在轉(zhuǎn)錄水平和蛋白水平的表達(dá),并通過分子檢測固定穩(wěn)定表達(dá)的抗性基因,并結(jié)合傳統(tǒng)育種系譜法選
8、擇具有優(yōu)良農(nóng)藝性狀株系.對選育的株系在
