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1、作物學(xué)報(bào)ACTAAGRONOMICASINICA2016,42(2):180?189http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.00180青稞遺傳多樣性及其農(nóng)藝性狀與SSR標(biāo)記的關(guān)聯(lián)分析1,21,21,21,21,21,2孟亞雄孟林祎汪軍成司二靜張海娟任盼榮1,21,31,21,2*馬小樂(lè)李葆春楊軻王化俊12甘肅省干旱生境作物學(xué)重點(diǎn)實(shí)驗(yàn)室/甘肅省作物遺傳改良與種質(zhì)
2、創(chuàng)新重點(diǎn)實(shí)驗(yàn)室,甘肅蘭州730070;甘肅農(nóng)業(yè)大學(xué)農(nóng)學(xué)院,甘肅3蘭州730070;甘肅農(nóng)業(yè)大學(xué)生命科學(xué)技術(shù)院,甘肅蘭州730070摘要:利用92個(gè)SSR標(biāo)記對(duì)108份青稞親本材料進(jìn)行多態(tài)性掃描,分析其遺傳多樣性,旨在尋找與農(nóng)藝性狀相關(guān)聯(lián)的分子標(biāo)記,為青稞雜交組合的配制及分子標(biāo)記輔助育種提供依據(jù)。挑選48個(gè)多態(tài)性標(biāo)記進(jìn)行群體遺傳結(jié)構(gòu)分析,在此基礎(chǔ)上采用Tassel2.1GLM(generallinearmodel)和MLM(mixedlinearmodel)方法進(jìn)行標(biāo)記與農(nóng)藝性狀的關(guān)聯(lián)分析。共檢測(cè)出156個(gè)等位變異,每個(gè)位點(diǎn)2~6個(gè)等位變
3、異。供試群體的Shannon指數(shù)為0.6727~1.1368,材料間遺傳相似系數(shù)為0.2250~1.0000,平均0.7585。通過(guò)群體遺傳結(jié)構(gòu)分析將供試材料劃分成4個(gè)亞群。以GLM分析,發(fā)現(xiàn)12個(gè)與株高、穗長(zhǎng)、穗粒數(shù)和分蘗數(shù)相關(guān)聯(lián)的標(biāo)記,對(duì)表型變異的解釋率分別為11.5%~17.6%、19.4%~45.4%、15.4%~22.1%和29.2%;以MLM分析,發(fā)現(xiàn)8個(gè)與株高、分蘗數(shù)和小穗數(shù)相關(guān)的標(biāo)記,各標(biāo)記對(duì)表型變異的解釋率分別為31.7%~49.8%、28.1%~37.2%、22.7%~32.7%。關(guān)聯(lián)標(biāo)記分布在基因組全部6個(gè)連鎖群上
4、。關(guān)鍵詞:青稞;SSR;遺傳多樣性;群體遺傳結(jié)構(gòu);關(guān)聯(lián)分析GeneticDiversityandAssociationAnalysisofAgronomicCharacteristicswithSSRMarkersinHullessBarley1,21,21,21,21,2MENGYa-Xiong,MENGYi-Lin,WANGJun-Cheng,SIEr-Jing,ZHANGHai-Juan,REN1,21,21,31,21,2,*Pan-Rong,MAXiao-Le,LIBao-Chun,YANGKe,andWANGHua-Jun1
5、GansuProvincialKeyLaboratoryofAridlandCropScience/GansuKeyLaboratoryofCropImprovement&GermplasmEnhancement,Lanzhou23730070,China;CollegeofAgronomy,GansuAgriculturalUniversity,Lanzhou730070,China;CollegeofLifeSciencesandTechnology,GansuAgriculturalUniversity,Lanzhou730070
6、,ChinaAbstract:Theobjectivesofthisstudyweretofindmolecularmarkersassociatedwithyield-relatedtraitsandguideparentalcombinationinmolecularmarker-assistedbreedingandhybridbreedingofhullessbarley(HordeumvulgareL.var.nudumHK.f.).Anaturalhullessbarleypopulationcomposedof108par
7、entalvarieties/lineswasscreenedwith92SSRmarkers,inwhich48markerswerepolymorphic.PopulationstructurewasanalyzedbasedonthepolymorphicSSRdataandassociationbetweenmarkersandfiveagronomictraitswereperformedinTASSELGLM(generallinearmodel)andMLM(mixedlinearmodel)programs.Atotal
8、of156allelesweredetectedinthe108varieties/lineswith2–6allelesperlocus.TheShannon’sindexofthepopulationr
