資源描述:
《高溫對(duì)喜樹(shù)生長(zhǎng)及葉片喜樹(shù)堿含量的影響》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、第40卷第3期南京林業(yè)大學(xué)學(xué)報(bào)(自然科學(xué)版)Vol.40,No.32016年5月JournalofNanjingForestryUniversity(NaturalSciencesEdition)May,2016doi:10.3969/j.issn.1000-2006.2016.03.004高溫對(duì)喜樹(shù)生長(zhǎng)及葉片喜樹(shù)堿含量的影響11112汪貴斌,周昕,劉琳,曹福亮,祖元?jiǎng)偅ǎ保戏浆F(xiàn)代林業(yè)協(xié)同創(chuàng)新中心,南京林業(yè)大學(xué)林學(xué)院,江蘇南京210037;2.東北林業(yè)大學(xué)林學(xué)院,森林植物生態(tài)學(xué)教育部重點(diǎn)實(shí)驗(yàn)室,黑龍江哈爾濱1
2、50040)摘要:喜樹(shù)堿是喜樹(shù)(Camptothecaacuminata)的一種次生代謝物,有良好的抗腫瘤活性,在喜樹(shù)體內(nèi)的合成和積累受遺傳和環(huán)境因子的影響。筆者在人工氣候室中研究了溫度對(duì)當(dāng)年生喜樹(shù)苗生長(zhǎng)及葉片喜樹(shù)堿合成的影響。試驗(yàn)設(shè)置了3種晝/夜(白天6:00至18:00,夜間18:00至次日6:00)溫度處理,分別為30℃/25℃(T1)、35℃/30℃(T2)和40℃/35℃(T3)。結(jié)果表明:T3溫度條件下,喜樹(shù)有最高的生物量,T2處理有利于喜樹(shù)苗高和地徑的生長(zhǎng),而T3處理則有利于喜樹(shù)根和莖的生物量積累
3、。T2處理促進(jìn)了喜樹(shù)葉片內(nèi)可溶性糖的積累,降低了可溶性蛋白質(zhì)的含量,而T1和T3處理則提高了葉片色氨酸脫羧酶(TDC)的活性;T2處理下喜樹(shù)堿含量和單株葉片喜樹(shù)堿產(chǎn)量最高。試驗(yàn)期間喜樹(shù)堿含量(產(chǎn)量)的變化趨勢(shì)與TDC活性的變化不一致。綜合認(rèn)為,適宜的溫度條件(T2)有利于喜樹(shù)體內(nèi)喜樹(shù)堿的生物合成與積累,喜樹(shù)堿在喜樹(shù)體內(nèi)的生物合成和積累是多種因素綜合作用的結(jié)果。關(guān)鍵詞:喜樹(shù);溫度;生長(zhǎng);喜樹(shù)堿;次生代謝中圖分類號(hào):S718;S792?99文獻(xiàn)標(biāo)志碼:A文章編號(hào):1000-2006(2016)03-0021-05E
4、ffectsoftemperatureonthegrowthandcamptothecincontentsofCamptothecaacuminata11112WANGGuibin,ZHOUXin,LIULin,CAOFuliang,ZUYuangang(1.Co?InnovationCenterforSustainableForestryinSouthernChina,CollegeofForestry,NanjingForestryUniversity,Nanjing210037,China;2.KeyLa
5、boratoryofForestPlantEcologyMinistryofEducation,CollegeofForestry,NortheastForestryUniversity,Harbin150040,China)Abstract:Camptothecin(CPT)isanimportantsecondarymetaboliteinCamptothecaacuminata,anditcanbeusedasananticancerdrug.Thebiosynthesisandaccumulationo
6、fCPTinC.acuminataareaffectedbyheredityandenvironment.Inthispaper,theeffectoftemperatureonthegrowthandCPTbiosynthesisofoneyearoldC.acuminatawasstudiedinphytotron.Threetemperatureday/night(daytime6:00-18:00,nighttime18:00-6:00),30℃/25℃(T1),35℃/30℃(T2)and40℃/30
7、℃(T3)weredesigned.WefoundthatC.acuminatapresentedthehighestbiomassatT3,andT2wasbeneficialtotheheightandcaliperofseedlings,andT3wasfavorabletotheaccumulationofstemandrootbiomass.T2treatmentpresentedthehighestsolublesugarcontent,butthelowestsolubleproteinconte
8、ntandtheactivityoftrypto?phandecarboxylas(TDC).AtT2condition,thehighestCPTcontentandCPTyieldperplantintheleaveswereob?served.ThechangingregulationofCPTcontentwasnotinaccordancewiththeactivityofT