資源描述：
《轉(zhuǎn)PvP5CS1 基因擬南芥植株對(duì)干旱和鹽脅迫的反應(yīng)》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、作物學(xué)報(bào)ACTAAGRONOMICASINICA2010,36(1):147?153http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2010.00147轉(zhuǎn)PvP5CS1基因擬南芥植株對(duì)干旱和鹽脅迫的反應(yīng)1,2211,*1,*陳吉寶趙麗英毛新國王述民景蕊蓮1中國農(nóng)業(yè)科學(xué)院作物科學(xué)研究所/農(nóng)作物基因資源與基因改良國家重大科學(xué)工程/農(nóng)業(yè)部作物種質(zhì)資源與生物技術(shù)重點(diǎn)實(shí)驗(yàn)室,2北京100081;南陽師范學(xué)院生命科學(xué)與
2、技術(shù)學(xué)院,河南南陽473061摘要:為探索普通菜豆脯氨酸合成酶基因P5CS1在植物滲透脅迫中的作用,本研究應(yīng)用農(nóng)桿菌介導(dǎo)法,將PvP5CS1基因轉(zhuǎn)入擬南芥,獲得6株陽性轉(zhuǎn)基因株系;通過檢測(cè)轉(zhuǎn)基因植株與野生型植株在干旱和鹽脅迫下種子發(fā)芽率,幼苗脯氨酸含量、株系電導(dǎo)率、相對(duì)根長和成株死亡率,分析了PvP5CS1基因的表達(dá)對(duì)改善擬南芥抗?jié)B透脅?1?1迫的效應(yīng)。結(jié)果表明,在150mmolLNaCl和150mmolL甘露醇滲透脅迫下,轉(zhuǎn)基因植株平均相對(duì)發(fā)芽率分別是?1?1野生型的1.60倍和1.62倍;150、250mmolL甘露醇和150mmolLNaCl處理下,轉(zhuǎn)基因擬南芥植株平
3、均脯氨酸含量分別是野生型的2.68、1.30和1.30倍;平均相對(duì)電導(dǎo)率分別是野生型植株的85%、77%和85%;平均相對(duì)根長?1分別是野生型植株的1.2、1.3和1.2倍;300mmolLNaCl處理下,轉(zhuǎn)基因植株的平均死亡率為42%,顯著低于野生型(90%)(P<0.05);干旱脅迫下,轉(zhuǎn)基因植株的平均死亡率為56%,顯著低于野生型(70%)(P<0.05),說明PvP5CS1基因在擬南芥中的表達(dá)明顯改善了轉(zhuǎn)基因植株的抗旱性和耐鹽性。關(guān)鍵詞:PvP5CS1;脯氨酸;干旱脅迫;鹽脅迫ResponseofPvP5CS1TransgenicArabidopsisPlantst
4、oDrought-andSalt-Stress1,2211,*1,*CHENJi-Bao,ZHAOLi-Ying,MAOXin-Guo,WANGShu-Min,andJINGRui-Lian1NationalKeyFacilityforCropGeneResourcesandGeneticImprovement/KeyLaboratoryofCropGermplasm&Biotechnology,Ministryof2Agriculture/InstituteofCropSciences,ChineseAcademyofAgriculturalSciences,Beijin
5、g100081,China;SchoolofLifeScienceandTechnology,NanyangNormalUniversity,Nanyang473061,ChinaAbstract:Underadverseenvironment,manyplantsincreasetheircellosmoticpotentialthroughaccumulationofintracellularorganicosmolytes-proline.Theprolineaccumulationinplantscannotonlyincreasecellosmoticpotent
6、ialbutalsostabilizepro-teins,membranes,andsubcellularstructuresaswellasprotectcellsagainstoxidativedamagebyreactiveoxygenspecies.Pyr-roline-5-carboxylatesynthetase(P5CS),arate-limitingenzymeinvolvedinthebiosynthesisofprolineinhigherplants,isencodedbyP5CSgene.ThefulllengthcDNAsequenceofPvP5
7、CS1genewassubclonedintopCHF3vectorandtransformedintowildArabidopsisviaAgrobacteriumtumefaciens.Atotalofsixpositivetransgenicplantswereobtained.TheresultofRT-PCRshowedthatthePvP5CS1genewasnormallyexpressedintransgenicplants.TheT4generationpurifiedlinesoftransge
