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1、Anal.Chem.2005,77,1496-1502ElectrochemicalImmunosensorsfortheSimultaneousDetectionofTwoTumorMarkersMichaelS.Wilson*EICLaboratories,Inc.,111DowneyStreet,Norwood,Massachusetts02062Themicrofabricationofelectrochemicalimmunosensorsinstrumentationisrelativelysi
2、mpleandcanbeminiaturizedeasilyforthesimultaneousdetectionoftwoproteinanalytesistocircuitboardlevelwithverylowpowerrequirements,facilitatingdescribed.Thesensorsconsistedoftwoiridiumoxidethedevelopmentofdisposabledevicesandmethodologiesforelectrodes(1-mmdiam
3、eter)patternedonaglasssub-ultrasmallsampleamounts.Whiletheuseofelectrochemicalstrate.Captureantibodieswereimmobilizedonthepo-methodsforperformingparallelsingle-analyteimmunoassayshasrousiridiumoxideelectrodesbycovalentattachmentbeenwell-established,3,4ther
4、ehasbeenlittleapplicationofsuchusing(3-aminopropyl)triethoxysilaneandglutaraldehyde.devicestoSMIA.ElectrochemicalmethodsforSMIAthatuseThespatialseparationoftheelectrodes(2.5mm)enabledmultipleenzymelabelshavebeenreported,5butthesemethodssimultaneouselectroc
5、hemicalimmunoassaystobecon-ofteninvolveacompromiseinassayconditionssincedifferentductedwithoutcross-talkbetweentheelectrodes.ProteinsenzymesmayhavedifferentrequirementsforoptimumenzymeweremeasuredusingelectrochemicalELISA,anddetec-activity.Furthermore,theu
6、seofmultiplelabelsincreasesthetionwasachievedbyelectrochemicallyoxidizingalkalinecomplexityandreducestheconvenienceofthesemethods.phosphatase-generatedhydroquinone.SensorsfortheAmoreattractivestrategyforelectrochemicalSMIAisoneinsimultaneousdetectionofgoat
7、IgGandmouseIgG,andwhichasingleenzymelabelisusedtodetectallanalytes.ThisforthetumormarkersCEAandAFP,weredeveloped.Themethodreliesonthespatialresolutionbetweendifferentantibodysensorshaddetectionlimitsof1,2,1.2,and1ng/mLandelectrodelocations.Inthisdesign,cro
8、ss-talkcanpotentiallyforgoatIgG,mouseIgG,CEA,andAFP,respectively.occurduetoelectroactiveproductgeneratedatoneelectrode,diffusingtoaneighboringelectrode,andproducinganinterferingSimultaneousmultianalyteimmunoa
