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《不同抗原修復(fù)法對(duì)視網(wǎng)膜石蠟切片相關(guān)功能蛋白表達(dá)檢測(cè)的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、眼科研究2010年1月第28卷第1期15實(shí)驗(yàn)研究不同抗原修復(fù)法對(duì)視網(wǎng)膜石蠟切片相關(guān)功能蛋白表達(dá)檢測(cè)的影響李穎胡運(yùn)韜馬汀馬志中TheeffectsofvariedantigenretrievalmethodsonimmunohistochemistryoffunctionalproteinsinretinalparaffinsectionLiYing,HuYuntao,MaTing,MaZhizhong.PekingUniversityThirdHospital,PekingU
2、niversityEyeCenter,Beijing100191,ChinaAbstractBackgroundAntigenretrievalmethodisthekeyofimprovingthesuccessfulrateofimmunohistochemicalassayinparaffinsections.Tostudyanavailablemethodofantigenretrievalisagoaltoachievebothgoodimmunochemistryresult
3、andpreservingretinalproteins.ObjectiveTheaimofpresentstudyistoinvestigatetyrosindigestion,hightemperatureheatpressure,waterbathheatingandmicrowaverepairinantigenretrievalforretinaltissues.MethodsRetinaltissuewasisolatedandobtainedfromcleanChinch
4、illarabbits.Fourhundredsretinalparaffinsectionswereprepared.Fourkindsofantigenretrievalmethodsforretinaltissueincludingtyrosindigestion,hightemperatureheatpressure,waterbathheatingandmicrowaverepairwereusedrespectively.Thedepigmentatedretinalparaffi
5、nsectionwithoutantigenretrievalwasusedascontro.lThepositiveratesofexpressionofCRALBP,Rhodopsinandopsinproteinswereevaluatedandcomparedamongfourkindsofantigenretrievalmethodsbyimmunochemistry.ResultsCRALBP,Rhodopsinandopsinproteinwaspositivelyexpres
6、sedincytoplasmofretinalpigmentepithelialcellsandtheoutersegmentofphotoreceptorrespectively.Nosignificantdifferencewasfoundinthepositiveexpressionratesofthesethreeproteinsamongfourkindsofantigenretrievalmethods(P>005),butthedifferencesintissueintegri
7、tyandbackgroundstainingwerestatisticallysignificant(P<001).Thestructuraldamageofretinaincludedlossandpuckerofscalera,crackofnucleusandabnormalbackgroundstaininhightemperatureheatpressuremethod,waterbathheatingmethodandmicrowaveretrievalmethod.Howev
8、er,stableCRALBP,Rhodopsinandopsinproteinexpressionandstraineffectiveness,clearbackgroundwithoutunspecificstainingandintegratedtissuewereseenintyrosindigestionmethod.ConclusionDuringtheclinicalpathologyanalysisofretinaltissue,theapplicationoftyrosin
