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1、中國醫(yī)科大學(xué)學(xué)報(bào)JChinMedUniv第34卷第4期2005年8月·301·高糖、糖基化終產(chǎn)物對大鼠主動脈平滑肌細(xì)胞巨噬細(xì)胞炎性蛋白1α表達(dá)的影響1213孟馨,商秀麗,張錦,劉中華(1.中國醫(yī)科大學(xué)附屬第一醫(yī)院內(nèi)分泌科,遼寧沈陽110001;2.附屬第一醫(yī)院神經(jīng)內(nèi)科;3.沈陽軍區(qū)司令部門診部)【摘要】目的:探討高糖濃度和糖基化終產(chǎn)物(AGEs)對大鼠主動脈平滑肌細(xì)胞巨噬細(xì)胞炎性蛋白(MIP)1αmRNA及蛋白表達(dá)的影響。方法:將培養(yǎng)的大鼠主動脈平滑肌細(xì)胞分別用不同濃度AGEs(100,200,400mg/L)單獨(dú)培養(yǎng)2
2、4h;400mg/LAGEs和22mmol/L葡萄糖聯(lián)合培養(yǎng)24h。RT2PCR方法及流式細(xì)胞儀檢測MIP21αmRNA及蛋白的表達(dá)。結(jié)果:對照組平滑肌細(xì)胞內(nèi)MIP21α呈弱表達(dá);100,200,400mg/LAGEs培養(yǎng)平滑肌細(xì)胞24h及22mmol/L葡萄糖和400mg/LAGEs聯(lián)合孵育24h后顯著增高M(jìn)IP21αmRNA的表達(dá),其電泳條帶相對積分吸光度值分別為對照組的1.36,1.75,2.45及2.76倍(P<0.05);各組平滑肌細(xì)胞MIP21α蛋白的平均熒光強(qiáng)度分別為對照組的1.24,1.63,2.37及2
3、.68倍(P<0.05)。結(jié)論:AGEs能刺激平滑肌細(xì)胞MIP21αmRNA及蛋白表達(dá)增加,且與高糖具有協(xié)同作用?！娟P(guān)鍵詞】葡萄糖;糖基化終產(chǎn)物;巨噬細(xì)胞炎性蛋白1α;平滑肌細(xì)胞,血管;大鼠【中圖分類號】Q49314【文獻(xiàn)標(biāo)識碼】A【文章編號】0258-4646(2005)04-0301-03EffectofhighconcentrationofglucoseandAGEsonthemRNAandproteinexpressionofmacrophageinflammatoryprotein21αinculturedVS
4、MCs1213MENGXin,SHANGXiu2li,ZHANGJin,LIUZhong2hua(1.DepartmentofEndocrinology,TheFirstAffiliatedHospital,ChinaMedicalUniversity,Shenyang110001,China;2.DepartmentofNeurologyTheFirstAffiliatedHospital;3.OutpatientDepartment,CommandofShenyangMilitaryArea)【Abstract】Ob
5、jective:Toinvestigatetheeffectofhighconcentrationofglucoseandadvancedglycationendprod2ucts(AGEs)onthemRNAandproteinexpressionofmacrophageinflammatoryprotein21α(MIP21α)inculturedvascularsmoothmusclecells(VSMCs).Methods:VSMCswereculturedwithdifferentconcentrationso
6、fACEs(100,200,and400mg/L)for24hoursandcoculturedwith22mmol/Lofglucoseand400mg/LofAGEsfor24hours.ThelevelsofmR2NAandproteinexpressionofMIP21αinculturedVSMCsweredetectedbyRT2PCRandflowcytometerrespectively.Re2sults:ThemRNAexpressionofMIP21αincreased1.36fold,1.75fol
7、d,2.45fold,and2.76fold,andtheproteinexpres2sionofMIP21αincreased1.24fold,1.63fold,2.37fold,and2.68foldinVSMCsculturedwithdifferentconcentrationsofAGEsandcoculturedwithglucoseandAGEscomparedwiththeBSAgroup(P<0.05).Theaveragefluorescentinten2sityvaluesofMIP21αwere1
8、97±3,260±3,375±6,and423±8inVSMCsand158±4inBSAgroup.Conclusion:AGEscouldinduceahighmRNAandproteinexpressionofMIP21αinculturedVSCMsinadose2dependentmanner.Therem
