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1、目錄摘要··········································································································1ABSTRACT··································································································4前言····································································
2、······································7第一部分當(dāng)歸多糖的提取、純化與鑒定·························································91.當(dāng)歸粗多糖的提取···················································································92.當(dāng)歸多糖的純化·······································································
3、··············103.當(dāng)歸多糖的鑒定·····················································································124.討論與小結(jié)····························································································17第二部分當(dāng)歸多糖肝靶向的研究·····························································
4、·····181.當(dāng)歸多糖標(biāo)熒光記物的制備·····································································192.當(dāng)歸多糖在大鼠肝實(shí)質(zhì)細(xì)胞和非實(shí)質(zhì)細(xì)胞中的分布·····································233.當(dāng)歸多糖在HEPG2細(xì)胞和HELA細(xì)胞中的攝入研究·····································324.討論與小結(jié)····························································
5、································34第三部分當(dāng)歸多糖對(duì)急性肝損傷保護(hù)作用體內(nèi)研究········································351.對(duì)乙酰氨基酚致大鼠急性肝損傷模型的建立···············································352.當(dāng)歸多糖對(duì)大鼠急性肝損傷保護(hù)作用藥效學(xué)研究········································393.當(dāng)歸多糖對(duì)大鼠急性肝損傷保護(hù)作用分子機(jī)制研究·······························
6、······474.討論與小結(jié)····························································································54第四部分當(dāng)歸多糖對(duì)急性肝損傷保護(hù)作用體外研究········································561.對(duì)乙酰氨基酚誘導(dǎo)肝細(xì)胞凋亡/壞死···························································562.當(dāng)歸多糖抑制肝細(xì)胞凋亡/壞死機(jī)制研究················
7、····································603.討論與小結(jié)····························································································67總結(jié)與討論··································································································68本論文創(chuàng)新點(diǎn)······················
