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1、AntonievanLeeuwenhoek79:377384,2001.377?2001KluwerAcademicPublishers.PrintedintheNetherlands.ResponseandtoleranceoftoxigenicVibrocholeraeO1tocoldtemperaturesJeffreyW.Carroll1,MadalinaC.Mateescu1,KiranChava1,RitaR.Colwell2,3&AsimK.Bej1,?1DepartmentofBiology,1300UniversityBo
2、ulevard,UniversityofAlabamaatBirmingham,Birmingham,AL35294-1170,USA;2DepartmentofCellandMolecularBiology,UniversityofMaryland,CollegePark,MD20742,USA;3CenterforMarineBiotechnology,UniversityofMarylandBiotechnologyInstitute,PrattStreet,Baltimore,MD21202-3101,USA(?Authorforc
3、orrespondence;Tel:+1-205-934-9857;Fax:+1-205-975-6097;E-mail:abej@uab.edu)Received15December;accepted15December2000Keywords:VibriocholeraeO1,coldadaptation,waterbornepathogen,choleratoxin,cspAAbstractSurvivalandtoleranceatcoldtemperatures,thedifferentiallyexpressedcellular
4、proteins,andcholeratoxin(CTX)productionwereevaluatedinVibriocholeraeO1.Rapidlossofculturabilityandchangetodistinctcoccoidmor-phologyoccurredwhenculturesofV.choleraeO1wereexposedto5?Cdirectlyfrom35?C.Also,culturesofV.cholerae?rstexposedto15?Cfor2handthenmaintainedat5?Cfaile
5、dtoexhibitanadaptiveresponse,insteadarapidlossofviableplatecountwasnoticed.ResultsfromWesternblotexperimentsrevealedtheabsenceofamajorcoldshockprotein,CS7.4.Also,adecreasedlevelofCTXwasnoticedinV.choleraeO1culturesexposedto5or15?Cafter?rstbeingexposedto15?Cfor2h,followedby
6、transferto5?C.ReducedexpressionofCTXatcoldtemperatures,comparedtotheculturesmaintainedat35?C,maybearesultofdecreasedcellularmetabolicactivity.WhenV.choleraeO1cultureswereexposedto15?Cfor2h,elevatedexpressionsof8,26and194kDa,anddecreasedexpressionof28and183kDaproteinsoccurr
7、ed.Itissuggestedthatthesedifferentiallyexpressedcold-responsiveproteinsareinvolvedinregulatingculturabilityandconversiontoacoccoidcellmorphologyinV.choleraeO1.IntroductionSeasonaldisappearanceoftoxigenicstrainsofV.choleraeO1frombothhumanpopulationsandfromVibriocholeraeO1,a
8、Gram-negativebacterium,istheenvironmentincholeraendemicregionshavethecausativeagentofchol
