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1、METHODSINMOLECULARMEDICINETMAtherosclerosisExperimentalMethodsandProtocolsEditedbyAngelaF.DrewHumanaPressAnimalModels11AnimalModelsofDiet-InducedAtherosclerosisAngelaF.Drew1.IntroductionAnimalsmodelsofatherosclerosisdeveloplesionseitherspontaneouslyorb
2、yinterventionssuchasdietary,mechanical,chemical,orimmunologicalinduction.Animalmodelsprovideameansforstudyingtheunderlyingmecha-nismsbehindtheatheroscleroticdiseaseprocess,aswellasameansforstudy-ingtheeffectofinterventions,dietaryorotherwise,onthedevel
3、opmentorregressionofdisease,whileundercontrolledconditions.Theeffectofriskfactorsforatheroscleroticdiseasedevelopmenthasbeenevaluatedinanimalmodels,withtheadvantageofexcludingotherinfluences.Animalmodelshaveprovidedvaluableinformationregardingdiagnosti
4、candtherapeuticstrategies,withextensiveinvestigationofeventsoccurringinthearterywallthroughouttheseprocedures.Animalmodelshaveprovidedinformationaboutfactorscontributingtodiseaseprogressionandregressionthatapplytohumansituations.Itisimportanttorecogniz
5、ethediversityofanimalmodelsthatexistforresearchandthevariousadvantagesordisadvantagesofeachmodelwhenchoosingthemostappropriatemodelforpotentialstudies.Thischapterprovidesinformationregardingthebenefitsanddisadvantagesofdiet-inducedmodelsofspontaneousat
6、herosclerosis.Becauseofthesuddenincreaseinpopu-larityofgeneticallymanipulatedmousemodels,furtherinformationispro-videdinalaterchapter.2.Models2.1.RabbitsRabbitshavebecomethemostpopularanimalmodelofatherosclerosis,withNewZealandWhite(NZW)rabbitsbeingthe
7、mostwidelyused.RabbitshaveFrom:MethodsinMolecularMedicine,vol.52:Atherosclerosis:ExperimentalMethodsandProtocolsEditedby:A.F.Drew?HumanaPressInc.,Totowa,NJ12Drewbeenusedinstudiesoflesioncharacterization,druginterventions,mechanicalarterialinjury,andart
8、erialmetabolism.Rabbitsaretypicallyfed0.5–2%cholesteroldietsfor4–16wk,dependingontheseverityofdiseaserequiredandthetimeavailableforinduction.Thisdietiswelltoleratedbyrabbits,andlesionsconsistentlyappear,thoughwithmarkedvariationinlesion
