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1、www.advmat.dewww.MaterialsViews.comCOMMUNICATIONAConvenientPreparationofMulti-SpectralMicroparticlesbyBacteria-MediatedAssembliesofConjugatedPolymerNanoparticlesforCellImagingandBarcodingXuliFeng,GaomaiYang,LibingLiu,*FengtingLv,QiongYang,ShuWang,*andD
2、aobenZhu*Recently,thedevelopmentofmultiplexedbioassaysandmolenanoparticles(CPNs)havebeenpreparedforcellimaging,cularimaginghasbecomeincreasinglyimportantingeneprowhichexhibithighfluorescencebrightness,excellentphotostafiling,drugdiscovery,andclinicaldi
3、agnostics.[1–3]Spectroscopicbilityandlowertoxicity.[20–24]However,thenumberofcolorsofcodingtechnologybasedonbothcolorandintensityoftheCPNsthemselvesislimited,anditisneededtofindaneffeclightemittedfrommorethanonecoloringmaterialsthataretivewaytoachievem
4、ulticolorregulationtomeettheneedsofembeddedinsideorattachedonthesurfaceofthemicropartimultiplexedbiologicalanalysisandimaging.AlthoughDNAcleshasbeendevelopedformultiplexedbioassays.[4?6]Oneofbasedselfassemblyforfluorescencenanobarcodeshavebeenthemajorl
5、imitationsisthatspecializedfiltersetsarerequiredstudied,[25,26]tothebestofourknowledgebacteriamediatedforeachdyebecauseorganicdyeshavewidelyseparatedabsorpselfassemblyformulticolorregulationhasnotbeeninvestitionandexcitationspectra.Toovercomethislimita
6、tionfluoresgatedbeforeformultiplexedbioassaysandimaging.Attenucenceresonanceenergytransfer(FRET)dyepairshavebeenatedintracellularbacteriaarebiocompatibleandbiodegradable,developed,[7–9]wherethepolymermicrospheresincorporateoneandhavebeenfoundtoactasgoo
7、dnonviralmeanstotransferdyedonorandoneormoredyeacceptorsthatundergoFRETplasmidDNAintomammaliancellsandtheirsurfacescanbefromdonortoacceptorstoproducevariedemissioncolorsoreasilymodifiedtoachieveantigenantibodyrecognition.[27]Inintensitiesunderasingleex
8、citationwavelength.ByFRET,largethispaper,wereportanoveltechniqueforpreparingencodedStokesshiftsareobtainedtoavoidbiologicalautofluorescencemulticolorCPNsbasedontheselfassemblyofbacteriaandandreducethebackgroundnoisesignal.However,fluore
