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1、萬(wàn)方數(shù)據(jù)·76史堡監(jiān)鏖匡垣鍪盍(皇±遂2絲!蘭生!壁旦筮壘鲞筮墊魍堡塹塾!墾墮查!i!塾璺(里!塑匭!型壅i堅(jiān))：堡叢墼!!：墊!蘭，!生：魚(yú)，№：墊化療聯(lián)合DC．CIK細(xì)胞治療對(duì)胰腺癌患者免疫功能的影響劉愛(ài)華【摘要】目的探討DC．CIK細(xì)胞免疫治療對(duì)化療后胰腺癌患者免疫功能的影響。方法對(duì)50例經(jīng)病理確診的胰腺癌患者依據(jù)治療方式分為兩組：化療聯(lián)合自身DC．CIK細(xì)胞治療25例患者作為聯(lián)合治療組，同期單純化療25例患者作為對(duì)照組。分別于治療前、治療后相同時(shí)間點(diǎn)取外周血，采用流式細(xì)胞儀檢測(cè)T細(xì)胞亞群及CIM+CD25+C
2、Dl27“調(diào)節(jié)性T細(xì)胞(Treg)的變化；ELISA方法檢測(cè)IFNl及IL-4水平的變化。結(jié)果聯(lián)合治療組患者治療后外周血CD3+、CD4+、CD3+CD56+T細(xì)胞百分率上升，CD4+／CD8+值上調(diào)，CD8+T細(xì)胞百分率下降(P<0．05或P<0．01)；CIM+CD25+CDl27”Treg下降(P<0．01)；n1細(xì)胞因子IFN．’水平較治療前升高，而Th2細(xì)胞因子I“水平降低(P<0．01)。對(duì)照組單純化療后CD3+、CIM+、CD8+細(xì)胞百分率下降(P<0．05或P<0．01)；CIM+CD25+CDl27
3、”Treg下降(P<0．01)；IFN一^y和IL4水平降低(P均<0．01)。結(jié)論化療聯(lián)合DC．CIK細(xì)胞治療能提高胰腺癌患者的免疫功能，增強(qiáng)機(jī)體的抗腫瘤免疫效應(yīng)，是治療胰腺癌患者的輔助手段之一?！娟P(guān)鍵詞】T淋巴細(xì)胞，調(diào)節(jié)性；細(xì)胞因子類；胰腺腫瘤；樹(shù)突狀細(xì)胞-細(xì)胞因子誘導(dǎo)的殺傷細(xì)胞InfluenceofchemotherapycombinedwithDC-CIKcellontheimmunefunctioninpatientswithpancreaticCallerUUAi—hua．Cer血越Laboratory。
4、TaianCentralHo印iml，Taian271000，ChinaEntail：liuaihua0698@12五com【Abstract】0bjectiveTostudytheeffectofdendriticceils—cytokineinducedkillercells(DC·CIK)immunotherapyafterchemotherapyonimmunityfunctionofpatientsofpancreaticcancer．Methods50patientswitllpathologically
5、confmmedpancreaticcancerwererandomlydividedinto2groups：combinedtherapygroup(25patientstreatedwithDC—CIKcelltherapyafterchemotherapy)andcontrolgroup(25patientstreatedwithchemotherapyonly)．鴨elevelsofTcellsubsetsCD3+，CIM+，CD8+，CD4+CD25+CDl27l*"regulatoryTcellswere
6、detectedwithflowcytometryandtheIFN-1andIL4weredetectedwithELISAinperipheralbloodbeforeandafterthetreatment．ResultsTcellsubsetsCD3+，CD4+andCD3+CD56+。ratioofCD4+／CD8+wereincreasedafterDC．CIKcelltransfusionandtheCD8+cellsubsetsandCIM+CIY25+CDl2710-regulatoryTceils
7、weresignificantlydecreased(P<0．05orP<0．01)．1helevelofIFN-1wereincreasedandthelevelofIL-4decreasedattheSanletime．Inthecontrolgroup，TcellsubsetsCD3+，CIM+，CD8+andCD4+CD25+CDl27t。"regulatoryTcellsweredecreasedaftertreatedwitIlchemotherapyonly(P<0．05orP<0。01)．Thelev
8、elsofIFN一叮andIL4weredecreased(P<0．01)．ConclusionsChemotherapycombinedwithDC-CIKcellimmunotherapyCanimprovetheimmunefunctionofpatientswithpancreaticcancerandenhancethebodyant
