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1、血清鐵調(diào)素在骨質(zhì)疏松模型中變化趨勢的觀察【摘要】目的研究SD大鼠維甲酸灌胃骨質(zhì)疏松模型中血清hepcidin含量的變化。方法取72只大鼠分為模型組和對照組，模型組以維甲酸70mg/(kg?d)持續(xù)灌胃14天，對照組等量蒸憾水灌胃14天，雙能X線骨密度儀器進行活體骨密度測定，模型組和對照組血清hepcidin含量EIJSA試劑盒進行測定。結(jié)果骨密度的測定顯示骨質(zhì)疏松模型組骨密度較對照組降低，差異有顯著性。模型組和對照組相比較，血清hepcidin含量在模型建立過程中有變化:初期略有降低,差異無顯著性；中期明顯降低，差異有顯著
2、性；后期略有回升，低于對照組，差異無顯著性。結(jié)論血清hepcidin含量變化與維甲酸致骨質(zhì)疏松形成可能存在直接或間接關(guān)系，hepcidin的變化可能成為骨質(zhì)礦化異常診斷、治療的新焦點，為進一步研究骨質(zhì)疏松提供新的思路?！娟P(guān)鍵詞】骨質(zhì)疏松維甲酸鐵調(diào)素血清ObservationofchangesofseroushepcidinconcentrationinSDratsosteoporosismodel[Abstract]ObjectiveToinvestigatethechangesofserumhepcidinconcent
3、rationinSDratsosteoporosismodelinducedbyintragasticadministrationwithtretinoin.Methods72SI)ratsweredividedintoexperimentalgroupandcontrolgrouprandomly,treatedtheexperimentalgroupwithtretinoinandthecontrolgroupwithdistilledwaterofequalquantitybyintragasticadminista
4、tionforlasted14days,assayedthebonedensitywithdualenergyX-rayabsorptiometry(DEXA)andserumhepcidinconcentrationofexperimentalgroupandcontrolgroupwithenzyme-linkedimmunosorbentassay(ELISA)?ResultsTheassayofbonedensityindicatedthatbonedensityofexperimentalgroupwasmuch
5、lowerthancontrolgroup?Incontrasttocontrolgroup,serumhepcidinconcentrationofexperimentalgroupchangedintheformationprocessofosteoporosismode1.Theserumhepcidinconcentrationdecreasedslightlywithnosignificantdifferenceatearlystage,decreasedobviouslywithsignificantdiffe
6、reneeatintermediatestage?Butincreasedslightlyatlatestage,sti111owerthanconcentrationofserumhepcidinofcontrolgroup,decreasewasofnostatisticsmeaning?ConclusionThechangesofserumhepcidinconcentrationmayhavedirectorindirectrelationwithosteoporosisformationinducedbyintr
7、agasticadministrationwithtretinoin.Perhapshepcidininvestigationwi11becomeanewfocuspointindiagnosisandtreatmentofbonemineralizationabnormalityandmakegreatprogressinstudyingosteoporosis.[Keywords]osteoporosis；tretinoin；hepcidin；serum近幾年來，許多研究結(jié)果認為體內(nèi)鐵代謝平衡和紊亂與骨質(zhì)礦化有著密切的
8、聯(lián)系。在研究絕經(jīng)女性骨質(zhì)疏松治療、預防時發(fā)現(xiàn)鐵的攝入與骨的礦化密度呈正相關(guān)［l,2］oDenisM.Medeiros等研究發(fā)現(xiàn)鐵缺乏飲食的骨礦化不良［3］oMargaretM.Harris等［4］研究結(jié)果顯示絕經(jīng)后婦女的飲食鐵攝入增加可以提高骨密度。鐵調(diào)素（hepcidin）是一種富含半胱氨酸的抗菌多肽
