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1、VirusResearch,2(198.5)53-5953ElsevierVRR00165BovinecoronavirushemagglutininproteinBernadetteKing,BarbaraJ.PottsandDavidA.Brian*DepartmentofMicrobiology,UniversityofTennessee,Knoxville,TN37916,U.S.A.(Accepted22October1984)Treatmentofpurifiedbovinecoronavirus(Mebusstrain)withpronasedestroyedt
2、heintegrityofvirionsurfaceglycoproteinsgp140,gp120,gp100,reducedtheamountofgp26anddestroyedthehemagglutinatingactivityofthevirus.Bromelain,ontheotherhand,destroyedtheintegrityofgp120,gplO0andgp26butfailedtoremovegp140andfailedtodestroyviralhemagglutinatingactivity.Theseexperi-mentssuggestth
3、atgp140isthevirionhema~uti~.I~~oblottingstudiesusingmonospecificantiserumdemonstratethatgp140isa~sul~de-~~dimericstruc-turereducibletomonomersof65kDa.bovinecoronavirus,hemagglutinin,bromelainresistanceBothhema~utinatingandno~e~~ut~atingmammaliancoronaviruseshavebeenidentified(McIntosh,1974)
4、.Whenseveralmammaliancoronaviruseswereclassifiedonthebasisofantigenicrelatednessitwasfoundthatthehemagglutinat-ingcoronavirusescouldbeclassedintooneoftwomajorantigenicsubgroups,althoughnotallviruseswithinthissubgrouphadhemagglutinatingproperties(Pedersonetal.,1978).Includedinthissubgroupwer
5、ethehemagglutinatingbovineentericcoronavirus,theporcinehemagglutinatingencephalomyelitisvirus,thehema~utinatinghumanrespiratorycoronavirus0C43,andtheno~ema~u~at-ingmousehepatitisvirus3.Inthispaperwereportthatthemoleculeresponsibleforhemagglutinationbythebovinecoronavirusisapparentlyoneoffou
6、rsurface$y;;roSns,gp140,whichexistsasadisulfide-linkeddimeroftwoglycoproteinsofPlaquepurifiedbovinecoronavirus(Mebusstrain)wasgrowneitheronthebovineembryokidneycelllineBEK-1(Inabaetal.,1976)oronthehumanrectaltumorcelllineHRT-18(Tompkinsetal,,1974fasdescribed(KingandBrian,1982).Virusgrownone
7、ithercelllinewasvirtuallyidenticalasjudgedfromelectrophoretic016%1702/85,/$03.3001985EkvierSciencePublishersB.V.(BiomedicalDivision)Fig.1.PropertiesoftheBCVgp140protein.[3H]Aminoacid-labeledBCV,purifiedasdescribed(KingandBrian,1982),waselectrophoresedona
