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1、J.gen.Virol.(1989),70,345-352.PrintedinGreatBritain345Keywords:BCV/haemagglutinin/processingSynthesisandProcessingoftheBovineEntericCoronavirusHaemagglutininProteinByBRENDAG.HOGUE,tTHOMASE.KIENZLEANDDAVIDA.BRIAN*DepartmentofMicrobiology,TheUniversityofTennessee,Knoxville,Tennessee37996-0845
2、,U.S.A.(Accepted26October1988)SUMMARYThehaemagglutininmoleculeonthebovineentericcoronavirushasbeenidentifiedasaglycoproteinof140Kcomposedofdisulphide-linkedsubunitsof65K.Inthisstudy,wehaveshownthesubunitstobeidenticalbydemonstratinganunambiguousamino-terminalaminoacidsequence.Theunglycosyla
3、tedsubunitwasfoundtohaveanMrof42.5Kandtoundergorapiddisulphidelinkageandglycosylation.Glycosylationwasfoundtobeoftheasparagine-linkedtypeandsomeoftheoligosaccharidesunderwentprocessingtocomplexforms.Studieswithinhibitorsofglycosylationsuggestedthataprocessingofthehaemagglutininoligosacchari
4、detakesplaceonthevirionwhilstitisintheGolgiapparatus.Eachhaemagglutininsubunitonthematurevirionwasestimatedtopossesssixorsevencarbohydratechainsofeitherthehigh-mannoseorhybridtype,andthreeorfourchainsofthecomplextype.INTRODUCTIONCoronaviruseshavebeengenerallydescribedaspossessingthreestruct
5、uralproteins:aphosphorylatednucleocapsidprotein(N)of50Kto60K,aglycosylatedmatrix-likeprotein(MorEl)of23Kto29K,andaglycosylatedpeplomericprotein(PorE2)of150Kto200K,thatinmanycasesisproteolyticallycleavedintotwosubunitsofroughlyequalsize(forreview,seeSiddelletal.,1982).Recently,afourthstructu
6、ralproteinhasbeendescribedasapartofthemammaliancoronavirusesthathaemagglutinate.Thesecoronavirusesincludethebovineentericcoronavirus(BCV)(King&Brian,1982),thehumanrespiratorycoronavirusOC43(Hogue&Brian,1986)andtheporcinehaemagglutinatingencephalomyelitisvirus(Callebaut&Pensaert,1980).Thefou
7、rthstructuralproteininBCVhasbeencharacterizedasaglycoproteinof140Kthatcomprisesdisulphide-linkedsubunitsof65K(King&Brian,1982).Itisthisproteinthatappearstobethevirionhaemagglutinin(H)(Kingetal.,1985).Consistentwiththispictureisthefindingthatthenon-haemag
