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1、ArchivesofVirologyArchivesofVirology68,35--44(1981)?bySpringer-Verlag1981InvivoMorpho9enesisoilaNewPorcineEntericCoronavirus,CV777By]~.DUCATELLE1,W.COUSSEMENT1,M.B.PENSAERT2,P.DEI~OUCK2,andJ.HOOREI~S1DepartmentsofPathologyIandVirology2,FacultyofVeterinaryMedicine,StateUniversityofGent,Gent,
2、BelgiumYVith9FiguresAcceptedNovember20,1980SmnmaryThemorphogenesisofanewporcineentericcoronavirus,CV777,inintestinalepithelialcellsofexperimentallyinfectednewbornpigletsisdescribed.ThevirusshowsamorphogeneticpatterncharacteristicformembersoftheCoronaviridaefamily.Itisformedbybuddingthroughm
3、embranesoftheendoplasmicreti-culum.Somespecificaspectsofthismorphogenesisarediscussed.IntroductionIn1978,anewcoronavirus-likeagent(CVLA),wasdemonstratedinthefaecesofpigletsfromoutbreaksofdiarrhoea(16).Theclinicalfindingsafternaturalandexperimentalinfectionhavebeende-scribed(7).Thevirushasbe
4、enprovisionallyclassifiedasacoronavirus,basedonitsmorphologicaspectwithnegativestaining.Ithasbeenshowntodiffersero-logicallyfromotherknownporcinecoronaviruses,transmissiblegastro-enteritisvirus(TGEV)andhaemagglutinatingencephalo-myelitisvirus(HEV)(16).Thepathogenesisoftheexperimentaloronasa
5、linfectionhasbeenstudiedbyimmunofluorescenee(8).TheaccompanyinghistologicalandultrastructurMlesionswillbereportedelsewhere(4,11).Theresultsofthesestudiesshowthatvirusreplicationoccursinthecytoplasmofabsorptiveepithelialcellscoveringthesmallintestinalvilliandthelargeintestine.Theintraeellula
6、rvirusreplicationresultsinalterationsofcellularorganellesandcelldesquamation.ThepresentstudydealswiththemorphogenesisoftheCVLAintheintestinalepithelialcellsofpigletsafterexperimentaloronasalinfection.MaterialsandMethodsSixteencaesarean-derivedcolostrum-deprivedpigletswereinoculatedoronasall
7、yonthesecondorthirddayoflifewith104piginfeetivedosesofavirusstock,obtained3*0304-8608/81/0068/0035/$02.0036R.DUCATELLEetal.:MorphogenesisofaNewPorcineEntericCoronavirusasdescribedelsewhere(DEBoUCKetal,tobepublished).Onepigletwaskeptasacontrol.Thepigswere
