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1、TheAdaptationofTwoHumanCoronavirusStrains(OC38andOC43)toGrowthinCellMonolayers(35068)MARIEBRUCKOV~~,~KENNETHMCTNTOSH,~ALBERT2.KAPIKIAN,ANDROBERTM.CHANOCKLabovaforyofInfectiousDiseases,NationalInstituteofAllergyandInfectiousDiseases,NafionalInstit?rtPsofHeaZth,Bethesda,Maryl
2、and20014Coronaviruses(1)ofatleasttwo,andThispaperreportsthesuccessfuladaptationprobablymore,serotypes(2)havebeenofstrainsOC38andOC43tomonolayersofrecoveredfromadultswithupperrespiratorymonkeyorigin.diseaseoccurringduringthewintermonthsMaterialsandMethods.Viruses.Coronavi-(3
3、-6).Serologicsurveyshaveshownthatrusstrainswiththeprefix“OC”wererecov-coronavirusinfectionismoderatelycommoneredinHETOCinthislaboratory(5).duringtheseperiodsofprevalence(6,7),StrainB814,recoveredinHETOC,waskind-andtheadministrationofcoronavirusestolyprovidedbyDr.D.A.J.Tyrre
4、ll(4).Allvolunteershasestablishedtheirpathogenicityorganculturegrownviruseswerepassagedforthehumanrespiratorytract(4,8).Re-morethanfourtimesinHETOCbeforeusesearchintotheirpropertieshas,however,inthesestudies.StrainsOC38andOC43beenhamperedbytheirfastidiousgrowthwereadaptedto
5、growthinsucklingmouserequirements.brainasdescribed(9).Fourteenofthe23reportedcoronavirusSera.Mouseserumhyperimmunetostrainstrainswereisolatedintissueculturemono-OC43waspreparedinweanlingCharlesRiverlayersofhumanorigin(3,6).AttemptstoCD-1Swissmice(9).PolyvalentMHVadaptthese1
6、4strainstogmwthinlaboratorymouseserumwaskindlyprovidedbyDr.animalswereunsuccessful.Theremaining9JohnC.ParkerofMicrobiologicAssociates.strainswereisolatedinhumanembryonicGuineapigserumhyperimmunetostraintrachealorganculture(HETOC)wherefur-229Ewaspreparedaspreviouslydescribed
7、therstudyhasprovedtobecumbersome(4,(3).5).Twostrains,OC38andOC43,weresuc-Tissueculture.Primaryvervetandrhesuscessfullyadaptedtogrowthinsucklingmicemonkeykidney(VMKandRhMK)andpri-(9).Inthissystemthestrainswereshowntomaryhumanembryokidney(HEK)tissuebeserologicallyidentical,an
8、daone-waycultureswereobtainedfromcommercialserologicrelationshipwithmousehepatitis
