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1、IMMUNOLOGICALINVESTIGATIONS,24(6),977-986(1995)INTERFERONyPOTENTIATESHUMANCORONAVIRUSOC43INFECTIONOFNEURONALCELLSBYMODULATIONOFHLACLASSIEXPRESSIONArleneR.CollinsDepartmentofMicrobiologySchoolofMedicine,StateUniversityofNewYorkatBuffaloBuffalo.NewYork14214ABST
2、RACTHCN-lA,ahumancerebralcorticalneuroncellline,wasexaminedforitssusceptibilitytohumancoronaviruses.The229estrainreplicatedefficiently,buttheOC43straindidnotreplicatewell,ifatall.Treatmentofthecellswithinterferon7at20U/mlfor48hrmarkedlyincreasedthesusceptibil
3、ityofthecellstoinfectionwithOC43virusasshownbya100-foldincreaseinsecretionofinfectiousvirusoverafourdayperiodascomparedtountreatedcontrols.TheincreasedsusceptibilitywasshowntobeduetomembraneexpressionofHLAclassIbyreceptor-blockadewithamonoclonalantibodyspecif
4、icforHLAmolecules.Forpersonaluseonly.INTRODUCTIONMultiplesclerosis(MS)ischaracterizedbyinflammatorydemyelinationofthecentralnervoussystem(CNS)andisbelievedtobeaneurologicaldiseaseofautoimmuneoriginforwhichaviraletiologyissuspected(1).Thecausativevirus(es)andi
5、tspathogenesisarestillundefined,butpersistentinfectionoftheCNSwiththevirusisthoughttobeinvolved.Theproductionofsubacuteandchronicdemyelinationinratsafterinfectionwithmousehepatitisvirus,type4(MHV-JHMstrain)hasledtothehypothesisthatthisgroupofvirusesmaybeassoc
6、iatedwithanMS-likediseaseofthecentralnervoussysteminman(2).EvidenceforImmunolInvestDownloadedfrominformahealthcare.combyChineseUniversityofHongKongon02/03/15anassociationcomesfromthefactthattwocoronaviruses,termedSKandSD,wereisolatedfromthebrainsoftwopatients
7、withmultiplesclerosis(3).Neutralizingantibodywasfoundinthecerebrospinalfluid(CSF)andserumofthepatientstotheisolatedviruses.The977Copyright01995byMarcelDrkker,Inc.978COLLINSisolatesarecloselyrelatedtomurinecoronavirusesandhumancoronavirusOC43bothserologicallya
8、ndbynucleicacidhybridization(4,5).Usingthereversetranscriptase-polymerasechainreaction(RT-PCR),murine-relatedcoronavirusRNAandhumancoronavirus229ebutnotOC43RNAwasfoundinagreaterpercentage
