資源描述：
《冠狀病毒Coronavirusl論文-1991 Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes_ a close genomic relati.pdf》由會(huì)員上傳分享，免費(fèi)在線(xiàn)閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、JournalofGeneralVirology(1991),72,1659-1666.PrintedinGreatBritain1659Sequenceanalysisoftheturkeyentericcoronavirusnucleocapsidandmembraneproteingenes:aclosegenomicrelationshipwithbovinecoronavirusArnoldVerbeekandPeterTijssen*CentredeRechercheenVirologie,lnstitutArmand-Frappier,Uni
2、versit~duQuebec.Laval-des-Rapides,QuebecH7V1B7,CanadaThe3'endoftheturkeycoronavirus(TCV)genomehypotheticalproteinof207aminoacidswithanMrofandthegeneencodingthenucleocapsidprotein(N)23KwasfoundwithintheNgenesequence.Theaminowereclonedandsequenced.Thegeneencodingtheacidsequencesofbo
3、ththeNandMproteinsweremoremembraneprotein(M)wasobtainedbycloningathan99%similartothosepublishedforBCV.polymerasechainreaction(PCR)-amplifiedfragmentExtensivesimilaritywasalsoobservedbetweentheobtainedusingbovinecoronavirus(BCV)-specificaminoacidsequencesoftheTCVNproteinandthosepri
4、mers.Furthermore,fiveTCVDNAfragments,ofmurinehepatitisvirus(MHV)(70%)andhumanobtainedbyPCRonRNAfromclinicalspecimensandrespiratorycoronavirusstrainOC43(HCV-OC43)correspondingtoeithertheNterminusoftheM(98%)andbetweentheaminoacidsequencesoftheproteinorthecompleteMproteinwerealsoclon
5、edandpredictedMproteinsofTCVandMHV(86%).Suchsequenced.Thesequencerevealeda3'non-codingstrikingidentitysuggeststhatBCV,TCVandHCV-regionof291bases,anopenreadingframe(ORF)OC43musthavedivergedfromeachotheronlyencodingtheNproteinwithapredictedsizeof448recently.ApotentialN-glycosylation
6、sitewasfoundataminoacids,oranMrof49K,andanORFencodingtheNterminusoftheTCVMproteinandissituatedattheMproteinwithapredictedsizeof230aminoacidsthesamelocationinBCV,MHVandtransmissibleandanMrof26K.AthirdORF,encodingagastroenteritisvirus.Introduction1985;Hogueetal.,1989;Parkeretal.,198
7、9),humanrespiratorycoronavirusstrainOC43(HCV-OC43;Ho-TheCoronaviridaefamilycontainsfourantigenicgroupsgue&Brian,1985),haemagglutinatingencephalitisvirus(Pedersonetal.,1978;Sturman&Holmes,1983).ofswine(Callebaut&Pensaert,1980),diarrhoeavirusofViruseswithineachgrouppossesspartialant
8、igenicinfantmice(Sugiyamaetal.,19
